Rabbit Anti-SOD2 Recombinant Antibody (clone 10F6)
CAT#: ZG-0575U
Rabbit Anti-SOD2 Antibody (clone 10F6) displayed high specificity against the SOD2 protein of Human and Rat.
Tested Data
Gene Expression
FC
Figure 1 Rabbit Anti-SOD2 Antibody (ZG-0575U) in FC
Western Blot
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, U87 whole cell lysate, K562 whole cell lysate, Rat brain tissue, Brain tissue, Liver tissue
All lanes: SOD2 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 25, 21, 19, 20 kDa
Observed band size: 25 kDa
WB
Figure 2 Rabbit Anti-SOD2 Antibody (ZG-0575U) in WB
IHC image of ZG-0575U diluted at 1:100 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1814/1865_C5_1_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_6_6.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_8_8.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_9_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2013/5464_A_7_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (BJ hTERT+, GAMG, HSkMC)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000112096-SOD2
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Specifications
- Immunogen
- A synthesized peptide derived from human SOD2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SOD2
- Species Reactivity
- Human, Rat
- Clone
- 10F6
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Microvascular Complications Of Diabetes 6 and Gastric Cancer.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOR-3370 | Hi-Affi™ Recombinant Rabbit Anti-SOD2 Monoclonal Antibody (DS3370AB) | WB, IHC, ICC | IgG |
| CAT | Product Name | Application | Type |
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| VS3-CJ334 | Mouse Anti-SOD2 Recombinant Antibody (VS3-CJ334) | WB, IHC, FC | |
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| VS7-0425-WR907 | Mouse Anti-SOD2 Recombinant Antibody (clone 8H3F9) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-0724-YC500 | AbPlus™ Anti-SOD2 Magnetic Beads (VS-0724-YC500) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
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| VS-0325-XY2142 | Anti-SOD2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6805 | Anti-Rat SOD2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6804 | Anti-Human SOD2 Immunohistochemistry Kit | IHC |
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