Rabbit Anti-SOX2 Antibody (VS-0924-YC91) - Cancer Stem Cell Marker (CAT#: VS-0924-YC91)
This product is an anti-SOX2 antibody (VS-0924-YC91) that targets the human SOX2 protein, a marker for cancer stem cells (CSCs). It is suitable for detecting SOX2 in CSC tissues or cells using Western blot (at a concentration of 0.1-0.5 µg/mL) and Immunofluorescence (at a concentration of 0.5-2 µg/mL).
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45725/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/45725/if_selected.jpg
Figure 2 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62851/1273_B4_7_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/62851/1273_B4_7_selected.jpg
Figure 3 High expression in cerebral cortex
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_9_5.jpg
(Glial cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_9_5.jpg
Figure 4 Esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_6_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_B_6_1.jpg
Figure 5 Colon
(Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_A_7_3.jpg
(Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/79745/167827_A_7_3.jpg
Figure 6 RNA cell line category: Cell line enhanced (AF22, HAP1, NTERA-2, U-251 MG)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181449-SOX2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181449-SOX2
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SOX2
- Species Reactivity
- Human
- Applications
- WB, IF
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot (WB): 0.1-0.5 µg/mL
Immunofluorescence (IF): 0.5-2 µg/mL
Target
- Alternative Names
- ANOP3; MCOPS3
- Gene ID
- 6657
- UniProt ID
- P48431
- Long Name
- SRY (sex determining region Y)-box 2
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
Methylation at Lys-42 and Lys-117 is necessary for the regulation of SOX2 proteasomal degradation.
- Protein Refseq
- NP_003097.1
- Function
- Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity).
Binds to the proximal enhancer region of NANOG (By similarity).
Critical for early embryogenesis and for embryonic stem cell pluripotency (PubMed:18035408).
Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity).
Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity).
May function as a switch in neuronal development (By similarity).
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Downloads
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Flow Cytometry (FC) related Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3377 | Hi-Affi™ Recombinant Rabbit Anti-SOX2 Monoclonal Antibody (DS3377AB) | ICC, IHC-P, WB, sELISA | IgG |
| MOR-0107-FY | Rabbit Anti-SOX2 Recombinant Antibody (clone AFY0078) | ICC, IHC-P, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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