Mouse Anti-SPA17 Recombinant Antibody (clone 3G9F3) (CAT#: VS7-0425-WR916)
This product is a mouse IgG1 antibody that binds to human SPA17. The antibody 3G9F3 recognizes specific amino acid domain of human SQSTM1 (AA: 232-356). It can be used for IHC and FC assays.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/if_selected.jpg
Figure 2 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/160496_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/160496_A_7_5.jpg
Figure 3 High expression in testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/160496_A_6_6.jpg
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/160496_A_6_6.jpg
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000064199-SPA17
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000064199-SPA17
Specifications
- Immunogen
- Recombinant human SP17 (AA: 1-152) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human SPA17
- Species Reactivity
- Human
- Clone
- 3G9F3
- Molecule Class
- Testis-Specific, Calmodulin-Binding Protein
- Applications
- IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Sperm Autoantigenic Protein 17; Cancer/Testis Antigen 22; Sperm Protein 17; SP17-1; SP17; CT22; Sperm Surface Protein Sp17
- Gene ID
- 53340
- UniProt ID
- Q15506
- Long Name
- Sperm Autoantigenic Protein 17
- Cellular Localization
- Membrane
- Protein Refseq
- NP_059121.1
- Function
- Sperm surface zona pellucida binding protein. Helps to bind spermatozoa to the zona pellucida with high affinity. Might function in binding zona pellucida and carbohydrates (By similarity).
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2428z-F(E) | Recombinant Anti-Human SPA17 Antibody Fab Fragment | FC, WB, FuncS | Fab |
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