Mouse Anti-SPA17 Recombinant Antibody (VS3-FY2880) (CAT#: VS3-FY2880)
This anti-SPA17 antibody is a mouse IgG recombinant monoclonal antibody. It has been specifically designed to target the human SPA17 protein, a calculated molecular weight of the protein is about 17.4 kDa. The optimal dilution for this antibody is Immunohistochemistry (1/50-1/200), Flow cytometry (1/50-1/200) and ELISA (1/1000-1/10000).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/if_selected.jpg
Figure 2 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/160496_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/160496_A_7_5.jpg
Figure 3 High expression in testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/160496_A_6_6.jpg
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/37568/160496_A_6_6.jpg
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000064199-SPA17
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000064199-SPA17
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human SPA17
- Species Reactivity
- Human
- Applications
- Immunohistochemistry, Flow cytometry, ELISA
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 17.4 kDa.
- Related Disease
- Testicular Cancer and Ovarian Cancer
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry: 1/50-1/200
Flow cytometry: 1/50-1/200
ELISA: 1/1000-1/10000
Target
- Alternative Names
- Sperm Autoantigenic Protein 17; Cancer/Testis Antigen 22; Sperm Protein 17; SP17-1; SP17; CT22; Sperm Surface Protein Sp17
- Gene ID
- 53340
- UniProt ID
- Q15506
- Cellular Localization
- Membrane
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (Q15506)
Modification sites at neXtProt (NX_Q15506)
Glycosylation from GlyGen (Q15506) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_024304351.1; XP_054225109.1; XP_054225110.1; NP_059121.1
- Function
- Sperm surface zona pellucida binding protein.
Helps to bind spermatozoa to the zona pellucida with high affinity.
Might function in binding zona pellucida and carbohydrates (By similarity).
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2428z-F(E) | Recombinant Anti-Human SPA17 Antibody Fab Fragment | FC, WB, FuncS | Fab |
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