CytoStream™ Rabbit Anti-SPI1 Recombinant Antibody (VS13-YC1105)
CAT#: VS13-YC1105
The CytoStream™ Rabbit Anti-SPI1 Recombinant Antibody is specific for human SPI1. SPI1 is an ETS-domain transcription factor activating genes in myeloid/B-lymphoid cells, binding purine-rich sequences, regulating gene expression and splicing. The anti-SPI1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line REH
Immunofluorescent staining of human cell line REH shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44653/1676_D3_2_selected.jpg
Normal Tissue
Figure 2 Bone marrow
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/44653/125448_B_6_4_val_selected.jpg
Normal Tissue
Figure 3 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_A_9_3.jpg
Normal Tissue
Figure 4 Skin
Fibrohistiocytic cells Staining: High Intensity: Strong Langerhans cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_B_9_1.jpg
Normal Tissue
Figure 5 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4079/11463_A_9_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HEL, HL-60, HMC-1, NB-4, THP-1, U-698, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000066336-SPI1
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Specifications
- Immunogen
- Recombinant human Spi1, aa 1-50.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SPI1
- Species Reactivity
- Human
- Applications
- WB, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Spi-1 Proto-Oncogene; Hematopoietic Transcription Factor PU.1; 31 KDa Transforming Protein; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene; 31 KDa-Transforming Protein; Transcription Factor PU.1;
- Gene ID
- 6688
- UniProt ID
- P17947
- Long Name
- Spi-1 Proto-Oncogene
- Sequence Similarities
- Belongs to the ETS family.
- Cellular Localization
- Cytosol
- Protein Refseq
- NP_001074016.1; NP_003111.2
- Function
- Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726).
Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity).
Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity).
May be particularly important for the pro- to pre-B cell transition (PubMed:33951726).
Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726).
In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SPI1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1727CT | Recombinant Mouse anti-Human SPI1 Monoclonal antibody (2G1) | ELISA, WB | |
| MRO-1297-CN | Recombinant Rabbit Anti-SPI1 Monoclonal Antibody (JB72-35) | WB, IHC, FC | Rabbit IgG |
| VS3-QX1049 | Mouse Anti-SPI1 Recombinant Antibody (clone 5C7) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-FY1861 | Rabbit Anti-SPI1 Recombinant Antibody (clone R07-4D3) | WB, IHC-P | Rabbit IgG |
| VS13-YC1104 | Rabbit Anti-SPI1 Recombinant Antibody (VS13-YC1104) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3386 | Hi-Affi™ Recombinant Rabbit Anti-SPI1 Monoclonal Antibody (DS3386AB) | FC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2162 | Anti-SPI1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY6860 | Anti-Human SPI1 Immunohistochemistry Kit | IHC |
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