CytoStream™ Rabbit Anti-SPI1 Recombinant Antibody (VS13-YC1105) (CAT#: VS13-YC1105)

• Immunogen
• Recombinant human Spi1, aa 1-50.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human SPI1

• Species Reactivity
• Human

• Applications
• WB, IHC-P, FC

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/1000
  Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
  Flow cytometry (FC): 1/50-1/100

• Alternative Names
• Spi-1 Proto-Oncogene; Hematopoietic Transcription Factor PU.1; 31 KDa Transforming Protein; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene Spi1; Spleen Focus Forming Virus (SFFV) Proviral Integration Oncogene; 31 KDa-Transforming Protein; Transcription Factor PU.1;

• Gene ID
• 6688

• UniProt ID
• P17947

• Long Name
• Spi-1 Proto-Oncogene

• Sequence Similarities
• Belongs to the ETS family.

• Cellular Localization
• Cytosol

• Protein Refseq
• NP_001074016.1; NP_003111.2

• Function
• Pioneer transcription factor, which controls hematopoietic cell fate by decompacting stem cell heterochromatin and allowing other transcription factors to enter otherwise inaccessible genomic sites. Once in open chromatin, can directly control gene expression by binding genetic regulatory elements and can also more broadly influence transcription by recruiting transcription factors, such as interferon regulatory factors (IRFs), to otherwise inaccessible genomic regions (PubMed:23658224, PubMed:33951726).
  Transcriptionally activates genes important for myeloid and lymphoid lineages, such as CSF1R (By similarity).
  Transcriptional activation from certain promoters, possibly containing low affinity binding sites, is achieved cooperatively with other transcription factors. FCER1A transactivation is achieved in cooperation with GATA1 (By similarity).
  May be particularly important for the pro- to pre-B cell transition (PubMed:33951726).
  Binds (via the ETS domain) onto the purine-rich DNA core sequence 5'-GAGGAA-3', also known as the PU-box (PubMed:33951726).
  In vitro can bind RNA and interfere with pre-mRNA splicing (By similarity).

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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