Mouse Anti-ssDNA Recombinant Antibody (clone 9F11) (CAT#: FAMAB-0067JF)
Recombinant monoclonal antibody can specially bind to ssDNA. This mouse anti-ssDNA antibody 9F11 can be considered a useful tool for the study of SLE.
Specific Inquiry
ELISA
Figure 1 Dose-response curves of 6 different sera obtained by ELISA for IgM antibodies against ssDNA.
Antibodies against denatured (ss) DNA of IgM class were demonstrated by ELISA.
Gripenberg, M., & Gripenberg, G. (1983). Expression of antibody activity measured by ELISA. Anti-ssDNA antibody activity characterized by the shape of the dose-response curve. Journal of immunological methods, 62(3), 315-323.
IHC
Figure 2 Fluorescence photomicrographs of MOLT-4 cells treated with 0.5 M sodium azide for 4 h.
Excitation: 450–490 nm. Magnification, 1×1000.
Frankfurt, O. S., Robb, J. A., Sugarbaker, E. V., & Villa, L. (1996). Monoclonal antibody to single-stranded DNA is a specific and sensitive cellular marker of apoptosis. Experimental cell research, 226(2), 387-397.
FC
Figure 3 Two-parameter contour plots of cells stained with MAb AP-13.
Note that S1 nuclease eliminated staining of apoptotic cells with the antibody.
Frankfurt, O. S., Robb, J. A., Sugarbaker, E. V., & Villa, L. (1996). Monoclonal antibody to single-stranded DNA is a specific and sensitive cellular marker of apoptosis. Experimental cell research, 226(2), 387-397.
Specifications
- Immunogen
- Hybridoma cells from 9F11
- Host Species
- Mouse
- Type
- Mouse IgG2b, κ
- Specificity
- Mouse ssDNA
- Species Reactivity
- Mouse
- Clone
- 9F11
- Applications
- ELISA, IHC, FC
- Related Disease
- SLE
Product Property
- Purity
- >95% as determined by SDS-PAGE and HPLC analysis
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- No preservatives
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- The ssDNA antibody has been reported in applications of Enzyme-linked Immunosorbent Assay, Flow Cytometry, Immunohistochemistry.
Target
- Alternative Names
- Single-stranded DNA; single-stranded deoxyribonucleic acid
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "Clone 9F11"
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Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-922 | Recombinant Anti-ssDNA Antibody | WB, ELISA, IHC, FuncS | IgG |
| PABZ-115 | Mouse Anti-ssDNA Recombinant Antibody (clone BV04-01) | WB, ELISA | Mouse IgG |
| MOR-A1340-YJ | Mouse Anti-ssDNA Recombinant Antibody (clone F7-26) | FC, ICC, IHC, IHC-P | Mouse IgM |
| MOR-A1341-YJ | Mouse Anti-ssDNA Recombinant Antibody (clone TNT-3) | ELISA, FC, ICC, IHC-P | Mouse IgG3 |
| MOR-A1365-YJ | Mouse Anti-ssDNA Recombinant Antibody (clone CBD505) | IF | Mouse IgG2a |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-922-F(E) | Recombinant Anti-ssDNA Antibody Fab Fragment | ELISA, WB, FuncS | Fab |
| PFBZ-115 | Mouse Anti-ssDNA Recombinant Antibody (clone BV04-01); Fab Fragment | WB, ELISA | Mouse Fab |
| PFBW-115 | Mouse Anti-ssDNA Recombinant Antibody (clone DNA-1); Fab Fragment | WB, IHC, IF, FuncS | Mouse Fab |
| FAMAB-0304CQ-F(E) | Mouse Anti-ssDNA Recombinant Antibody (clone BXW-DNA14); Fab Fragment | ELISA | Mouse Fab |
| FAMAB-0070WJ-F(E) | Human Anti-ssDNA Recombinant Antibody (clone 16/6); Fab Fragment | ELISA | Human Fab |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0347MC | Recombinant Human Anti-ssDNA antibody | IHC, IP, FC |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0304CQ-S(P) | Mouse Anti-ssDNA Recombinant Antibody (clone BXW-DNA14); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0305CQ-S(P) | Mouse Anti-ssDNA Recombinant Antibody (clone BXW-DNA16); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0306CQ-S(P) | Mouse Anti-ssDNA Recombinant Antibody (clone BXW-DNA7); scFv Fragment | ELISA | Mouse scFv |
| FAMAB-0059WJ-S(P) | Human Anti-ssDNA Recombinant Antibody (clone 7B3); scFv Fragment | ELISA, Inhib | Human scFv |
| FAMAB-0067JF-S(P) | Mouse Anti-ssDNA Recombinant Antibody (clone 9F11); scFv Fragment | WB, ELISA, FuncS | Mouse scFv |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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