Mouse Anti-SUFU Recombinant Antibody (VS3-FY2884)
CAT#: VS3-FY2884
The anti-SUFU product has been developed to bind human SUFU protein. It cross-reacts with african green monkey, mouse species. The calculated molecular weight of the protein is about 53.9 kDa. The monoclonal antibody can be used in the area of Joubert Syndrome 32 and Basal Cell Nevus Syndrome 2.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8700/43_C10_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human adrenal gland
Immunohistochemical staining of human adrenal gland shows strong nuclear and cytoplasmic positivity in cortical cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8700/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Glial cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8700/24413_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8700/24413_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8700/24413_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Nuclear
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8700/24413_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Preleptotene spermatocytes
Staining: Low
Intensity: Moderate
Quantity: <25%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity: 75%-25%
Sertoli cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8700/24413_A_5_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8700/24413_A_9_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000107882-SUFU/subcellular
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human, African Green Monkey and Mouse SUFU
- Species Reactivity
- Human, African Green Monkey, Mouse
- Applications
- Western Blot
- Conjugate
- Unconjugated, FITC
- MW
- The molecular weight of the target protein is approximately 53.9 kDa.
- Related Disease
- Joubert Syndrome 32 and Basal Cell Nevus Syndrome 2
Product Property
- Clonality
- Monoclonal Antibody
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/2000
Target
- Alternative Names
- Suppressor of fused homolog, SUFUH, SUFU
- Gene ID
- 51684
- UniProt ID
- Q9UMX1
- Sequence Similarities
- Belongs to the SUFU family.
- Cellular Localization
- Cytoplasm
Nucleus
- Post Translation Modifications
- Polyubiquitinated at Lys-257 by the SCF(FBXL17) complex, leading to its subsequent degradation and allowing the release of GLI1 for proper hedgehog/smoothened signal transduction (PubMed:27234298).
Ubiquitination is impaired by phosphorylation at Ser-342, Ser-346, Ser-352 and Thr-353 (PubMed:27234298).
(Q9UMX1-SUFU_HUMAN)
Phosphorylation at Ser-342, Ser-346, Ser-352 and Thr-353 prevents ubiquitination by the SCF(FBXL17) complex. (Q9UMX1-SUFU_HUMAN)
Ubiquitination at Lys257 and Lys321 (NX_Q9UMX1 [NX_Q9UMX1-1])
Modification sites at PhosphoSitePlus
- Protein Refseq
- XP_011538162.1; XP_011538163.1; XP_011538165.1; XP_011538166.1
- Function
- May also act by linking GLI1 to BTRC and thereby targeting GLI1 to degradation by the proteasome (PubMed:10559945, 10564661, 10806483, 24217340).
Sequesters GLI1, GLI2 and GLI3 in the cytoplasm, this effect is overcome by binding of STK36 to both SUFU and a GLI protein (PubMed:10559945, 10564661, 10806483, 24217340).
Negative regulator of beta-catenin signaling (By similarity).
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Related Signaling Pathways
Hedgehog Signaling Pathway
JAK-STAT Signaling Pathway
Related Diseases
Pancreatic Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "SUFU"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3458 | Hi-Affi™ Recombinant Rabbit Anti-SUFU Monoclonal Antibody (DS3458AB) | FC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2204-CN | Rabbit Anti-SUFU Polyclonal Antibody (MRO-2204-CN) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1217 | Rabbit Anti-SUFU Recombinant Antibody (VS3-WK1217) | IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1218 | Mouse Anti-SUFU Recombinant Antibody (VS3-WK1218) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY495 | Rabbit Anti-NHP SUFU Recombinant Antibody (VS-1024-XY495) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2225 | Anti-SUFU Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0126-XL1390 | Rabbit Anti-Sufu (phospho S342) Antibody | Phospho Antibodies for Cell Signaling Research |
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