Rabbit Anti-SUZ12 Recombinant Antibody (clone 1B10) (CAT#: ZG-0588U)
Rabbit Anti-SUZ12 Antibody (clone 1B10) displayed high specificity against the SUZ12 protein of Human.
Specific Inquiry
WB
Figure 1 Rabbit Anti-SUZ12 Antibody (ZG-0588U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, HL60 whole cell lysate, THP-1 whole cell lysate, K562 whole cell lysate
All lanes: SUZ12 antibody at 1:2000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 84 kDa
Observed band size: 90 kDa
IHC
Figure 2 Rabbit Anti-SUZ12 Antibody (ZG-0588U) in IHC
IHC image of ZG-0588U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IP
Figure 3 Rabbit Anti-SUZ12 Antibody (ZG-0588U) in IP
Immunoprecipitating SUZ12 in K562 whole cell lysate
Lane 1: Rabbit control IgG instead of ZG-0588U in K562 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: ZG-0588U(2µg)+ K562 whole cell lysate(500µg)
Lane 3: K562 whole cell lysate (10µg)
Specifications
- Immunogen
- A synthesized peptide derived from human SUZ12
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human SUZ12
- Species Reactivity
- Human
- Clone
- 1B10
- Applications
- ELISA, WB, IHC, IP
- Conjugate
- Unconjugated
- Related Disease
- Imagawa-Matsumoto Syndrome and Endometrial Stromal Sarcoma.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IP:1:200-1:1000
Target
- Alternative Names
- Polycomb protein SUZ12; Chromatin precipitated E2F target 9 protein; ChET 9 protein; Joined to JAZF1 protein; Suppressor of zeste 12 protein homolog; SUZ12; CHET9 JJAZ1 KIAA0160
- Gene ID
- 23512
- UniProt ID
- Q15022
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3472 | Hi-Affi™ Recombinant Rabbit Anti-SUZ12 Monoclonal Antibody (DS3472AB) | WB, ICC/IF, IP | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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