Mouse Anti-TARDBP Recombinant Antibody (clone 7F9A6)

Name: Mouse Anti-TARDBP Recombinant Antibody (clone 7F9A6)
SKU: VS7-0425-WR948
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR948

This product is a mouse IgG1 antibody that binds to human TARDBP. The antibody 7F9A6 recognizes specific amino acid domain of human TARDBP (AA: 126-260). It can be used for FC assay.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line A-431

Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/138_C12_2_selected.jpg

Subcellular Location and Protein Expression Figure 2 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/662_G6_1_selected.jpg

Subcellular Location and Protein Expression Figure 3 IHC staining of human epididymis

Immunohistochemical staining of human epididymis shows distinct positivity in both nuclei and cytoplasm of glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 4 IHC staining of human pancreas

Immunohistochemical staining of human pancreas shows strong nuclear positivity in exocrine glandular cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/ihc_selected.jpg

Subcellular Location and Protein Expression Figure 5 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/17284/166_C12_1_red_green.jpg

Normal Tissue Figure 6 Cerebral cortex

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_B_8_5.jpg

Normal Tissue Figure 7 Colon

Endothelial cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_3.jpg

Normal Tissue Figure 8 Liver

Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_4.jpg

Normal Tissue Figure 9 Kidney

Cells in glomeruli
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_8_5.jpg

Normal Tissue Figure 10 Testis

Leydig cells
Staining:High
Intensity: Strong
Quantity:>75%
Pachytene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Preleptotene spermatocytes
Staining:High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining:High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining:High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_6_6.jpg

Normal Tissue Figure 11 Lymph node

Germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Nuclear

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/3703/10479_A_9_8.jpg

RNA Expression Figure 12 RNA cell line category: Low cell line specificity

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000120948-TARDBP

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Specifications

Immunogen
Recombinant human TARDBP (AA: 126-260) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human TARDBP

Species Reactivity
Human

Clone
7F9A6

Molecule Class
Rna-Binding Protein

Applications
FC

Conjugate
Unconjugated

Related Disease
Amyotrophic lateral sclerosis 10 (ALS10)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
TARDBP; TDP43; ALS10; TAR DNA-Binding Protein 43; TAR DNA Binding Protein; TDP-43

Gene ID
23435

UniProt ID
Q13148

Long Name
TAR DNA binding protein

Cellular Localization
Cytoplasm; Mitochondrion; Nucleus

Post Translation Modifications
Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation

Protein Refseq
NP_031401.1

Function
DNA-binding; Repressor; RNA-binding

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR948.pdf

MSDS

MSDS - VS7-0425-WR948.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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