Rabbit Anti-TBX21 Recombinant Antibody (clone R07-7Y4) (CAT#: VS3-XY3520)

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human TBX21

• Species Reactivity
• Human

• Clone
• R07-7Y4

• Applications
• IHC-P, FC

• Related Disease
• Asthma; Nasal Polyps; Aspirin Intolerance; Immunodeficiency 88

• Clonality
• Monoclonal

• Aggregation
• < 5%

• Format
• Liquid

• Buffer
• PBS (pH 7.4), 150 mM NaCl and 50% glycerol

• Preservative
• 0.02% sodium azide

• Storage
• Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• IHC: 1/50-1/100
  FC: 1/50-1/100

• Alternative Names
• TBET; IMD88; T-PET; T-bet; TBLYM

• Gene ID
• 30009

• UniProt ID
• Q9UL17

• Long Name
• T-box transcription factor 21

• Cellular Localization
• Nucleus

• Post Translation Modifications
• Phosphorylations at Ser-53, Tyr-77, Ser-225 and Ser-513 are regulated by mTORC1.
  Phosphorylation at Tyr-530 is essential for its interaction GATA3.
  Phosphorylation at Tyr-220, Tyr-266 and Tyr-305 enhances its transcriptional activator activity.
  Phosphorylation at Thr-303 is required for its interaction with NFATC2.

• Protein Refseq
• NP_037483.1

• Function
• Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931).
  Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3.
  Induces permissive chromatin accessibilty and CpG methylation in IFNG (PubMed:33296702).
  Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation.

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Secondary Antibody

• 2RD-A1473-YJ
• Goat Anti-Rabbit IgG-F(ab')2 Secondary antibody

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