Rabbit Anti-TBX21 Recombinant Antibody (clone R07-7Y4) (CAT#: VS3-XY3520)
The recombinant rabbit antibody is specific for TBX21 (T-box transcription factor 21), which activates transcription of a set of genes important for Th1 cell function. TBX21 also inhibits the Th17 cell lineage commitment by blocking RUNX1-mediated transactivation of Th17 cell-specific transcriptinal regulator RORC. The antibody specifically reacts with human TBX21. It has been tested using Immunohistochemistry-Paraffin and Flow cytometry. The recommended dilutions of Immunohistochemistry-Paraffin and Flow cytometry is 1/50-1/100.
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Normal Tissue
RNA Expression
Figure 1 Spleen
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9524/24853_B_7_4_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9524/24853_B_7_4_val_selected.jpg
Figure 2 Liver
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_A_8_4.jpg
(Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_A_8_4.jpg
Figure 3 Bone marrow
(Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_B_5_4.jpg
(Hematopoietic cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_B_5_4.jpg
Figure 4 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_A_9_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/30043/70685_A_9_8.jpg
Figure 5 RNA cell line category: Cell line enriched (HDLM-2)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073861-TBX21
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000073861-TBX21
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TBX21
- Species Reactivity
- Human
- Clone
- R07-7Y4
- Applications
- IHC-P, FC
- Related Disease
- Asthma; Nasal Polyps; Aspirin Intolerance; Immunodeficiency 88
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- IHC: 1/50-1/100
FC: 1/50-1/100
Target
- Alternative Names
- TBET; IMD88; T-PET; T-bet; TBLYM
- Gene ID
- 30009
- UniProt ID
- Q9UL17
- Long Name
- T-box transcription factor 21
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Phosphorylations at Ser-53, Tyr-77, Ser-225 and Ser-513 are regulated by mTORC1.
Phosphorylation at Tyr-530 is essential for its interaction GATA3.
Phosphorylation at Tyr-220, Tyr-266 and Tyr-305 enhances its transcriptional activator activity.
Phosphorylation at Thr-303 is required for its interaction with NFATC2.
- Protein Refseq
- NP_037483.1
- Function
- Lineage-defining transcription factor which initiates Th1 lineage development from naive Th precursor cells both by activating Th1 genetic programs and by repressing the opposing Th2 and Th17 genetic programs (PubMed:10761931).
Activates transcription of a set of genes important for Th1 cell function, including those encoding IFN-gamma and the chemokine receptor CXCR3.
Induces permissive chromatin accessibilty and CpG methylation in IFNG (PubMed:33296702).
Activates IFNG and CXCR3 genes in part by recruiting chromatin remodeling complexes including KDM6B, a SMARCA4-containing SWI/SNF-complex, and an H3K4me2-methyltransferase complex to their promoters and all of these complexes serve to establish a more permissive chromatin state conducive with transcriptional activation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Related Resources
Inflammatory Bowel Diseases
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibodies
Intrabodies
Rabbit Monoclonal Antibodies
IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3561z | Mouse Anti-TBX21 Recombinant Antibody (clone 29A4) | WB, IP, IF, ELISA | Mouse IgG1, κ |
| MOB-1929CT | Recombinant Mouse anti-Human TBX21 Monoclonal antibody (39D) | ELISA, WB | |
| MRO-2210-CN | Rabbit Anti-Tbx21 Polyclonal Antibody (MRO-2210-CN) | WB, FC | Rabbit IgG |
| VS13-YC1142 | Rabbit Anti-TBX21 Recombinant Antibody (VS13-YC1142) | IHC-P | Rabbit IgG |
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