Mouse Anti-TFAP2A Recombinant Antibody (clone 7D2B5)
CAT#: VS7-0425-WR968
This product is a mouse IgG1 antibody that binds to human TFAP2A. The antibody 7D2B5 recognizes specific amino acid domain of human TFAP2A (AA: 1-100) . It can be used for IHC and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56871/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/326/if_selected.jpg
Normal Tissue
Figure 3 Placenta
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/56871/170334_A_1_7_val_selected.jpg
Normal Tissue
Figure 4 Kidney
Collecting ducts Staining: Medium Intensity: Strong Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/326/170388_A_7_5.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (HDLM-2, hTCEpi, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000137203-TFAP2A
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Specifications
- Immunogen
- Recombinant human TFAP2A (AA: 1-100) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human TFAP2A
- Species Reactivity
- Human, Mouse, Monkey, Rat
- Clone
- 7D2B5
- Molecule Class
- Transcription Factor
- Applications
- IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Branchiooculofacial syndrome (BOFS)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- Transcription Factor AP-2 Alpha; Transcription Factor AP-2 Alpha (Activating Enhancer Binding Protein 2 Alpha); Activating Enhancer-Binding Protein 2-Alpha; AP-2 Transcription Factor; Activator Protein 2; AP2-ALPHA; AP2TF
- Gene ID
- 7020
- UniProt ID
- P05549
- Long Name
- Transcription Factor AP-2 Alpha
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001027451.1
- Function
- Activator; DNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0625MZ | Recombinant Mouse Anti-Human TFAP2A Antibody (clone B7/3/3) | ELISA, IHC-P, WB | Mouse antibody |
| CAT | Product Name | Application | Type |
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| MOR-3537 | Rabbit Anti-TFAP2A Recombinant Antibody (clone DS3537AB) | WB, IHC, ICC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
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| VS3-QX1110 | Mouse Anti-TFAP2A Recombinant Antibody (clone 3C1-H7-G9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS3-QX1112 | Mouse Anti-TFAP2A Recombinant Antibody (clone 10-C5) | WB, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS3-XY1501 | Mouse Anti-TFAP2A Recombinant Antibody (clone 4A9D6) | ELISA, WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
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| VS-1024-XY28 | Rabbit Anti-NHP TFAP2A Recombinant Antibody (VS-1024-XY28) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2270 | Anti-TFAP2A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7182 | Anti-Mouse TFAP2A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7181 | Anti-Human TFAP2A Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
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