Rabbit Anti-TFG Recombinant Antibody (clone R06-8F5)
CAT#: VS3-FY1915
This R06-8F5 recombinant monoclonal antibody is specifically engineered to target human TFG. It cross-reacts with mouse, rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. This TFG antibody was verified by western blot analysis of TRK fused gene in C6, 3T3 lysates and immunohistochemistry analysis of paraffin-embedded human tonsil.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19473/234_A2_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52206/if_selected.jpg
Normal Tissue
Figure 3 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19473/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19473/44456_B_8_5.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19473/44456_A_9_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19473/44456_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19473/44456_A_4_6.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000114354-TFG
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WB
Figure 1 Anti-TFG Antibody (VS3-FY1915) in WB.
The TRK fused gene expression in C6, 3T3 lysates were detected using the anti-TFG by western blot assay.
IHC
Figure 2 Anti-TFG Antibody (VS3-FY1915) in IHC.
Immunohistochemistry staining of paraffin-embedded human tonsil using the anti-TRK fused gene antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for antigen retrieval.
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Specifications
- Immunogen
- Synthetic peptide of human TRK fusion gene
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TFG
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-8F5
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 43 kDa, while the observed molecular weight stands at 43 kDa.
- Related Disease
- Spastic Paraplegia 57, Autosomal Recessive and Neuropathy, Hereditary Motor And Sensory, Okinawa Type
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- TRK-Fused Gene; TRK-Fused Gene Protein; TRKT3 Oncogene; Protein TFG; HMSNP; SPG57; TRKT3; TF6
- Gene ID
- 10342
- UniProt ID
- Q92734
- Cellular Localization
- Endoplasmic reticulum
- Post Translation Modifications
- Ubiquitination at Lys47 and Lys103 (NX_Q92734 [NX_Q92734-1])
Modification sites at PhosphoSitePlus (Q92734)
Glycosylation from GlyGen (Q92734) 9 sites, 1 O-linked glycan (9 sites)
- Protein Refseq
- XP_005247123.1; XP_006713535.1; XP_011510636.1; XP_016861016.1; XP_047303197.1
- Function
- Plays a role in the normal dynamic function of the endoplasmic reticulum (ER) and its associated microtubules.
Required for secretory cargo traffic from the endoplasmic reticulum to the Golgi apparatus.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TFG"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3546 | Rabbit Anti-TFG Recombinant Antibody (clone DS3546AB) | WB, IHC-P, FC, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1529-CN | Rabbit Anti-TFG Recombinant Antibody (clone CBACN-559) | WB, IF, IHC, IP | Rabbit IgG |
| VS3-WK1084 | Rabbit Anti-TFG Recombinant Antibody (VS3-WK1084) | WB, ICC, IF, IHC, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7196 | Anti-TFG Immunohistochemistry Kit | IHC |
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