Rabbit Anti-THBD Recombinant Antibody (clone R08-4C6)
CAT#: VS3-FY1601
The R08-4C6 recombinant monoclonal antibody has been meticulously designed to target human THBD. It is suitable for use in a variety of assays, including WB, ICC/IF, IP. The specificity of the thrombomodulin antibody was confirmed by western blot analysis of thrombomodulin in Jurkat lysates.
Gene Expression
Tested Data
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/29_E4_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong membranous positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human placenta
Immunohistochemical staining of human placenta shows luminal positivity.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2982/30_E4_1_red_green.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/6389_A_7_3.jpg
Normal Tissue
Figure 6 Placenta
Trophoblastic cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2425/6389_A_3_7.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (ASC diff, CAPAN-2, EFO-21, HaCaT, hTCEpi, OE19, THP-1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000178726-THBD
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WB
Figure 1 Anti-THBD Antibody (VS3-FY1601) in WB.
The Thrombomodulin expression in Jurkat lysates were detected using the anti-Thrombomodulin (THBD) by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human thrombomodulin
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human THBD
- Species Reactivity
- Human
- Clone
- R08-4C6
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation
- MW
- The calculated weight of the target protein is approximately 60 kDa, while the observed molecular weight stands at 95 kDa.
- Related Disease
- Thromboembolic disease
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Target
- Alternative Names
- Thrombomodulin; CD141 Antigen; Fetomodulin; THRM; TM; THPH12; AHUS6; BDCA3; CD141
- Gene ID
- 7056
- UniProt ID
- P07204
- Cellular Localization
- Membrane
- Post Translation Modifications
- N-glycosylated., The iron and 2-oxoglutarate dependent 3-hydroxylation of aspartate and asparagine is (R) stereospecific within EGF domains.
- Protein Refseq
- NP_000352.1
- Function
- Thrombomodulin is a specific endothelial cell receptor that forms a 1:1 stoichiometric complex with thrombin. This complex is responsible for the conversion of protein C to the activated protein C (protein Ca). Once evolved, protein Ca scissions the activated cofactors of the coagulation mechanism, factor Va and factor VIIIa, and thereby reduces the amount of thrombin generated.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY207 | CytoStream™ Rat Anti-THBD Recombinant Antibody (VS-0225-XY207) | FC | Rat IgG2a, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC711 | Recombinant Anti-THBD Vesicular Antibody, EV Displayed (VS-0425-YC711) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7228 | Anti-THBD Immunohistochemistry Kit | IHC | |
| VS-0525-XY7229 | Anti-Mouse THBD Immunohistochemistry Kit | IHC | |
| VS-0525-XY7231 | Anti-Rat THBD Immunohistochemistry Kit | IHC |
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