Rabbit Anti-THEMIS Recombinant Antibody (clone R37-2V-5) (CAT#: VS3-FY2135)
Clone R37-2V-5 is a rabbit IgG recombinant monoclonal antibody that is specifically engineered to target human THEMIS protein, a calculated weight of 73 kDa. It cross-reacts with mouse, rat. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HDLM-2
(Immunofluorescent staining of human cell line HDLM-2 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/1962_H8_3_selected.jpg
(Immunofluorescent staining of human cell line HDLM-2 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/1962_H8_3_selected.jpg
Figure 2 Tonsil
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/ihc_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/ihc_selected.jpg
Figure 3 Colon
(Enterocytes - Microvilli Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_7_3.jpg
(Enterocytes - Microvilli Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_7_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_8_5.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_7_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_7_8.jpg
Figure 6 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4, RPMI-8226)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172673-THEMIS
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172673-THEMIS
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat THEMIS
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R37-2V-5
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 73 kDa, while the observed molecular weight stands at 73kDa.
- Related Disease
- Developmental Coordination Disorder and Lynch Syndrome 1
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/50
Flow Cytometry: 1/50
Target
- Alternative Names
- Thymocyte Selection Associated; Thymocyte-Expressed Molecule Involved In Selection; Thymocyte Selection Pathway Associated; C6orf190; C6orf207; Thymocyte Expressed Molecule Involved In Selection; Signaling Phosphoprotein Specific For T Cells; Chromosome 6 Open Reading Frame 207
- Gene ID
- 387357
- UniProt ID
- Q8N1K5
- Sequence Similarities
- Belongs to the themis family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated on Tyr residues quickly after TCR stimulation. (Q8N1K5-THMS1_HUMAN)
Ubiquitination at Lys124, Lys92, Lys208, Lys226, Lys451, and Lys595 (NX_Q8N1K5 [NX_Q8N1K5-1])
Modification sites at PhosphoSitePlus (Q8N1K5)
Glycosylation from GlyGen (Q8N1K5) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047274719.1; XP_047274720.1; XP_047274721.1; XP_047274722.1; XP_047274723.1
- Function
- Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3561 | Rabbit Anti-THEMIS Recombinant Antibody (clone DS3561AB) | WB, ICC | Rabbit IgG |
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