Rabbit Anti-THEMIS Recombinant Antibody (clone R37-2V-5) (CAT#: VS3-FY2135)
Clone R37-2V-5 is a rabbit IgG recombinant monoclonal antibody that is specifically engineered to target human THEMIS protein, a calculated weight of 73 kDa. It cross-reacts with mouse, rat. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/50), flow cytometry (dilution 1/50).
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line HDLM-2
(Immunofluorescent staining of human cell line HDLM-2 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/1962_H8_3_selected.jpg
(Immunofluorescent staining of human cell line HDLM-2 shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/1962_H8_3_selected.jpg
Figure 2 Tonsil
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/ihc_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/ihc_selected.jpg
Figure 3 Colon
(Enterocytes - Microvilli Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_7_3.jpg
(Enterocytes - Microvilli Staining: Medium Intensity: Moderate Mucosal lymphoid cells Staining: Low Intensity: Moderate Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_7_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_8_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_8_5.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_7_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/31422/151678_A_7_8.jpg
Figure 6 RNA cell line category: Group enriched (HDLM-2, JURKAT, MOLT-4, RPMI-8226)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172673-THEMIS
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000172673-THEMIS
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat THEMIS
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R37-2V-5
- Applications
- Western Blot, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 73 kDa, while the observed molecular weight stands at 73kDa.
- Related Disease
- Developmental Coordination Disorder and Lynch Syndrome 1
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/50
Flow Cytometry: 1/50
Target
- Alternative Names
- Thymocyte Selection Associated; Thymocyte-Expressed Molecule Involved In Selection; Thymocyte Selection Pathway Associated; C6orf190; C6orf207; Thymocyte Expressed Molecule Involved In Selection; Signaling Phosphoprotein Specific For T Cells; Chromosome 6 Open Reading Frame 207
- Gene ID
- 387357
- UniProt ID
- Q8N1K5
- Sequence Similarities
- Belongs to the themis family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylated on Tyr residues quickly after TCR stimulation. (Q8N1K5-THMS1_HUMAN)
Ubiquitination at Lys124, Lys92, Lys208, Lys226, Lys451, and Lys595 (NX_Q8N1K5 [NX_Q8N1K5-1])
Modification sites at PhosphoSitePlus (Q8N1K5)
Glycosylation from GlyGen (Q8N1K5) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_047274719.1; XP_047274720.1; XP_047274721.1; XP_047274722.1; XP_047274723.1
- Function
- Plays a central role in late thymocyte development by controlling both positive and negative T-cell selection. Required to sustain and/or integrate signals required for proper lineage commitment and maturation of T-cells. Regulates T-cell development through T-cell antigen receptor (TCR) signaling and in particular through the regulation of calcium influx and phosphorylation of Erk.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3561 | Rabbit Anti-THEMIS Recombinant Antibody (clone DS3561AB) | WB, ICC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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