Rabbit Anti-TNFAIP3 Recombinant Antibody (clone R06-9B2)
CAT#: VS3-FY1908
Clone R06-9B2 is a rabbit recombinant monoclonal antibody that effectively recognizes human TNFAIP3. The antibody is recommended for detection of TNFAIP3 protein by WB, IHC-F, IHC-P, ICC/IF. The TNFAIP3 antibody is guaranteed for western blot analysis of TNFAIP3 in Hela lysates and immunohistochemistry analysis of paraffin-embedded human lung cancer tissue.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2116/if_selected.jpg
Normal Tissue
Figure 2 IHC staining of human lymph node
Immunohistochemical staining of human lymph node shows cytoplasmic positivity in subse of germinal center and non-germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2116/8974_A_8_8_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2116/8974_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2116/8974_A_7_3.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2116/8974_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2116/8974_A_5_6.jpg
Normal Tissue
Figure 7 Bone marrow
Hematopoietic cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2116/8974_B_4_4.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (GAMG, Karpas-707, REH, U-266/84)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000118503-TNFAIP3
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WB
Figure 1 Anti-TNFAIP3 Antibody (VS3-FY1908) in WB.
The TNFAIP3 expression in Hela lysates were detected using the anti-TNFAIP3 by western blot assay.
IHC
Figure 2 Anti-TNFAIP3 Antibody (VS3-FY1908) in IHC.
Immunohistochemistry staining of paraffin-embedded human lung cancer tissue using the anti-TNFAIP3 antibody. High-pressure and temperature Sodium Citrate pH 6.0 was used for.
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Specifications
- Immunogen
- Synthetic peptide of human TNFAIP3
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TNFAIP3
- Species Reactivity
- Human
- Clone
- R06-9B2
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 90 kDa, while the observed molecular weight stands at 82 kDa.
- Related Disease
- Human lung cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- TNF Alpha Induced Protein 3; Putative DNA-Binding Protein A20; OTU Domain-Containing Protein 7C; Zinc Finger Protein A20; OTUD7C; Tumor Necrosis Factor, Alpha-Induced Protein 3; Tumor Necrosis Factor, Alpha Induced Protein 3; Tumor Necrosis Factor Alpha-Induced Protein 3
- Gene ID
- 7128
- UniProt ID
- P21580
- Sequence Similarities
- Belongs to the peptidase C64 family.
- Cellular Localization
- Cytoplasm, Lysosome, Nucleus
- Post Translation Modifications
- Proteolytically cleaved by MALT1 upon TCR stimulation; disrupts NF-kappa-B inhibitory function and results in increased IL-2 production.
It is proposed that only a fraction of TNFAIP3 colocalized with TCR and CBM complex is cleaved, leaving the main TNFAIP3 pool intact.
(P21580-TNAP3_HUMAN)
Ubiquitination at Lys541, Lys621, Lys643, Lys676, and Lys722 (NX_P21580 [NX_P21580-1])
Modification sites at PhosphoSitePlus (P21580)
Glycosylation from GlyGen (P21580) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005267176.1; XP_011534397.1; XP_011534398.1; XP_024302300.1; XP_024302301.1
- Function
- Deubiquitinates TRAF6 probably acting on 'Lys-63'-linked polyubiquitin. Upon T-cell receptor (TCR)-mediated T-cell activation, deubiquitinates 'Lys-63'-polyubiquitin chains on MALT1 thereby mediating disassociation of the CBM (CARD11:BCL10:MALT1) and IKK complexes and preventing sustained IKK activation. Deubiquitinates NEMO/IKBKG; the function is facilitated by TNIP1 and leads to inhibition of NF-kappa-B activation. Upon stimulation by bacterial peptidoglycans, probably deubiquitinates RIPK2. Can also inhibit I-kappa-B-kinase (IKK) through a non-catalytic mechanism which involves polyubiquitin; polyubiquitin promotes association with IKBKG and prevents IKK MAP3K7-mediated phosphorylation.
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Related Signaling Pathways
TNF Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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|---|---|---|---|
| MOB-4021z | Mouse Anti-TNFAIP3 Recombinant Antibody (clone 24G10) | WB, FC, ICC, IF, IHC, IP | Mouse IgG1, κ |
| MOB-2068CT | Recombinant Mouse anti-Human TNFAIP3 Monoclonal antibody (60B537) | FC, IHC-P, WB | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0595MZ | Chicken Anti-TNFAIP3 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3603 | Rabbit Anti-TNFAIP3 Recombinant Antibody (clone DS3603AB) | FC, ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2315 | Anti-TNFAIP3 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7358 | Anti-Mouse TNFAIP3 Immunohistochemistry Kit | IHC | |
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