Rabbit Anti-TNFRSF1B Recombinant Antibody (clone R36-5A-4)
CAT#: VS3-FY2119
Clone R36-5A-4 is a rabbit recombinant monoclonal antibody that is specifically created to bind human TNFRSF1B. TNFRSF1B protein has a calculated weight of 48 kDa. It cross-reacts with mouse, rat. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20), flow cytometry (dilution 1/50).
Gene Expression
Normal Tissue
Figure 1 IHC staining of human stomach
Immunohistochemical staining of human stomach shows positivity in plasma.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/14125_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_6_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (CAPAN-2, K-562, THP-1, U-87 MG, U-937)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000028137-TNFRSF1B
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TNFRSF1B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R36-5A-4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 48 kDa, while the observed molecular weight stands at 73kDa.
- Related Disease
- Classic Mycosis Fungoides and Sezary's Disease
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Flow Cytometry: 1/50
Target
- Alternative Names
- TNF Receptor Superfamily Member 1B; Tumor Necrosis Factor Receptor Type II; Tumor Necrosis Factor Receptor 2; P80 TNF-Alpha Receptor; TNF-RII; TNF-R2; TNFR2; TNFBR; P75; Tumor Necrosis Factor Receptor Superfamily, Member 1B; Tumor Necrosis Factor Receptor Superfamily Member 1B; Tumor Necrosis Factor Binding Protein 2; Tumor Necrosis Factor Beta Receptor
- Gene ID
- 7133
- UniProt ID
- P20333
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- Phosphorylated; mainly on serine residues and with a very low level on threonine residues. (P20333-TNR1B_HUMAN)
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. (P20333-TNR1B_HUMAN)
Glycosylation at Thr30, Asn171, Asn193, Thr206, Ser208, Ser221, and Thr222 (NX_P20333 [NX_P20333-1])
Modification sites at PhosphoSitePlus (P20333)
Glycosylation from GlyConnect TNR1B_HUMAN (705)
Glycosylation from GlyGen (P20333) 10 sites, 6 O-linked glycans (8 sites)
- Protein Refseq
- XP_011540362.1; XP_011540365.1; XP_047285378.1; XP_047285379.1; XP_047285380.1
- Function
- Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS3-FY2119. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TNFRSF1B"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2054z | Mouse Anti-TNFRSF1B Recombinant Antibody (clone 33A8) | WB, ELISA, Neut | Mouse IgG1 |
| MOR-0014-FY | Mouse Anti-TNFRSF1B Recombinant Antibody (clone MR2-1) | FC, FuncS, IA, IF, IP, WB | Mouse IgG1 |
| MOR-0018-FY | Mouse Anti-TNFRSF1B Recombinant Antibody (clone 80M2) | FC, FuncS, IA, IF, IP | Mouse IgG1 |
| HPAB-1550WJ | Mouse Anti-TNFRSF1B Recombinant Antibody (clone 32) | ELISA, WB | Mouse IgG1 |
| VS-0824-YJ45 | Anti-TNFRSF1B Recombinant Antibody (VS-0824-YJ45) | ELISA, WB, Inhib |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2153CQ | Rat Anti-TNFRSF1B Recombinant Antibody (NEUT-2153CQ) | FC, Neut | Rat IgG2a, κ |
| NEUT-2155CQ | Hamster Anti-Tnfrsf1b Recombinant Antibody (NEUT-2155CQ) | FC, IP, Block | Hamster IgG |
| NEUT-2156CQ | Mouse Anti-TNFRSF1B Recombinant Antibody (clone CBL430) | WB, ELISA, Neut | Mouse IgG1 |
| NEUT-2157CQ | Mouse Anti-TNFRSF1B Recombinant Antibody (clone CBL431) | ICC, Neut | Mouse IgG2a |
| NEUT-2158CQ | Rabbit Anti-TNFRSF1B Recombinant Antibody (clone CBL1000) | Neut | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-2154CQ | Hamster Anti-Tnfrsf1b Recombinant Antibody (clone TR75-54.7) | FC, IP, Block, WB, ELISA | Hamster IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3615 | Rabbit Anti-TNFRSF1B Recombinant Antibody (clone DS3615AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0245-WJ-S(P) | Mouse Anti-TNFRSF1B Recombinant Antibody; scFv Fragment (HPAB-0245-WJ-S(P)) | ELISA, FC | Mouse scFv |
| HPAB-1550WJ-S(P) | Mouse Anti-TNFRSF1B Recombinant Antibody; scFv Fragment (clone 32) | ELISA, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0245-WJ-F(E) | Mouse Anti-TNFRSF1B Recombinant Antibody; Fab Fragment (HPAB-0245-WJ-F(E)) | ELISA, FC | Mouse Fab |
| HPAB-1550WJ-F(E) | Mouse Anti-TNFRSF1B Recombinant Antibody; Fab Fragment (clone 32) | ELISA, WB | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY49 | Mouse Anti-TNFRSF1B Agonistic Antibody (clone MR2-1) | FC, IHC, Agonistic assays | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1501 | AbPlus™ Anti-Tnfrsf1b Magnetic Beads (VS-0724-YC1501) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC599 | Recombinant Anti-TNFRSF1B Vesicular Antibody, EV Displayed (VS-0425-YC599) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7381 | Anti-TNFRSF1B Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: FC, Cyt, Stim, PP, Agonist
Application: WB, ELISA, IP, FC, FuncS, Neut, IF
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
-2.png)
Application: WB, ELISA, FC, IHC, IP
Application: WB, IHC, ELISA, FC
Application: WB, ELISA, FC, IHC, IF, IP
Application: FC, IHC, Cyt, FuncS
Application: IF, ICC, WB, IHC-P, IP
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
