Rabbit Anti-TNFRSF1B Recombinant Antibody (clone R36-5A-4) (CAT#: VS3-FY2119)
Clone R36-5A-4 is a rabbit recombinant monoclonal antibody that is specifically created to bind human TNFRSF1B. TNFRSF1B protein has a calculated weight of 48 kDa. It cross-reacts with mouse, rat. The recommend antibody dilution is determined as western blot (dilution 1/500-1/1000), immunohistochemistry (dilution 1/100-1/200), immunofluorescence (dilution 1/50-1/200), immunoprecipitation (dilution 1/20), flow cytometry (dilution 1/50).
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows positivity in plasma.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/ihc_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/14125_B_8_5.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glial cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/14125_B_8_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_9_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_5.jpg
(Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_6_6.jpg
Figure 7 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4796/13963_A_8_8.jpg
Figure 8 RNA cell line category: Cell line enhanced (CAPAN-2, K-562, THP-1, U-87 MG, U-937)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000028137-TNFRSF1B
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000028137-TNFRSF1B
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TNFRSF1B
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R36-5A-4
- Applications
- Western Blot, Immunohistochemistry-Paraffin, Immunofluorescence, Immunoprecipitation, Flow Cytometry
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 48 kDa, while the observed molecular weight stands at 73kDa.
- Related Disease
- Classic Mycosis Fungoides and Sezary's Disease
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Immunofluorescence: 1/50-1/200
Immunoprecipitation: 1/20
Flow Cytometry: 1/50
Target
- Alternative Names
- TNF Receptor Superfamily Member 1B; Tumor Necrosis Factor Receptor Type II; Tumor Necrosis Factor Receptor 2; P80 TNF-Alpha Receptor; TNF-RII; TNF-R2; TNFR2; TNFBR; P75; Tumor Necrosis Factor Receptor Superfamily, Member 1B; Tumor Necrosis Factor Receptor Superfamily Member 1B; Tumor Necrosis Factor Binding Protein 2; Tumor Necrosis Factor Beta Receptor
- Gene ID
- 7133
- UniProt ID
- P20333
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- Phosphorylated; mainly on serine residues and with a very low level on threonine residues. (P20333-TNR1B_HUMAN)
A soluble form (tumor necrosis factor binding protein 2) is produced from the membrane form by proteolytic processing. (P20333-TNR1B_HUMAN)
Glycosylation at Thr30, Asn171, Asn193, Thr206, Ser208, Ser221, and Thr222 (NX_P20333 [NX_P20333-1])
Modification sites at PhosphoSitePlus (P20333)
Glycosylation from GlyConnect TNR1B_HUMAN (705)
Glycosylation from GlyGen (P20333) 10 sites, 6 O-linked glycans (8 sites)
- Protein Refseq
- XP_011540362.1; XP_011540365.1; XP_047285378.1; XP_047285379.1; XP_047285380.1
- Function
- Receptor with high affinity for TNFSF2/TNF-alpha and approximately 5-fold lower affinity for homotrimeric TNFSF1/lymphotoxin-alpha. The TRAF1/TRAF2 complex recruits the apoptotic suppressors BIRC2 and BIRC3 to TNFRSF1B/TNFR2. This receptor mediates most of the metabolic effects of TNF-alpha.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2054z | Mouse Anti-TNFRSF1B Recombinant Antibody (clone 33A8) | WB, ELISA, Neut | Mouse IgG1 |
| MOB-1590MZ | Recombinant Mouse Anti-Human TNFRSF1B Antibody (clone 33332.422) | ELISA, Neut, WB | Mouse antibody |
| MOR-0014-FY | Mouse Anti-TNFRSF1B Recombinant Antibody (clone MR2-1) | FC, FuncS, IA, IF, IP, WB | Mouse IgG1 |
| MOR-0018-FY | Mouse Anti-TNFRSF1B Recombinant Antibody (clone 80M2) | FC, FuncS, IA, IF, IP | Mouse IgG1 |
| HPAB-1550WJ | Mouse Anti-TNFRSF1B Recombinant Antibody (clone 32) | ELISA, WB | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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