Mouse Anti-TNFSF18 Recombinant Antibody (clone YGL386) (CAT#: FAMAB-1344CQ)

Figure 1 Cell surface expression of mGITRL.

(A) Spleen cells were stained with anti-mGITRL antibody YGL386 (solid line) or an isotype control antibody (dotted line). These cells were costained with anti-CD3, anti-B220, or anti-F4/80 antibody and then positive cells were gated. Median fluorescence intensity (MFI) is as follows: B220+ B cell (control, 7.7; mGITRL, 14.9), F4/80+ macrophages (control, 25.4; mGITRL low, 56.2; mGITRL high, 673.2), and CD3+ T cell (control, 11.2; mGITRL, 9.65). (B) Peritoneal cells were also stained with anti-mGITRL antibody YGL386 (solid line) or an isotype control antibody (dotted line). Cells were costained with anti-F4/80 antibody and then positive cells were gated. MFI are: control, 254.8 and mGITRL, 421.7. (C) Nonstimulated (0 h) and LPS-stimulated (6, 12, and 24 h) bmDCs were stained with anti-mGITRL antibody YGL 386 (solid line) or an isotype control antibody (dotted line). bmDC were costained with an anti-CD11c antibody (DC maker), and positive cells were gated. MFI values are indicated under the histograms.

Tone, M., Tone, Y., Adams, E., Yates, S. F., Frewin, M. R., Cobbold, S. P., & Waldmann, H. (2003). Mouse glucocorticoid-induced tumor necrosis factor receptor ligand is costimulatory for T cells. Proceedings of the National Academy of Sciences, 100(25), 15059-15064.