Rabbit Anti-TOP2A Recombinant Antibody (clone 5B10) (CAT#: ZG-0598U)
Rabbit Anti-TOP2A Antibody (clone 5B10) displayed high specificity against the TOP2A protein of Human and Mouse.
Specific Inquiry
WB
Figure 1 Rabbit Anti-TOP2A Antibody (ZG-0598U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: TOP2A antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 175, 178, 179, 183 kDa
Observed band size: 175 kDa
IHC
Figure 2 Rabbit Anti-TOP2A Antibody (ZG-0598U) in IHC
IHC image of ZG-0598U diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
Specifications
- Immunogen
- A synthesized peptide derived from human Topoisomerase II alpha
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TOP2A
- Species Reactivity
- Human, Mouse
- Clone
- 5B10
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Malignant Peripheral Nerve Sheath Tumor and Female Breast Cancer.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- DNA topoisomerase 2-alpha; EC 5.99.1.3; DNA topoisomerase II; alpha isozyme; TOP2A; TOP2
- Gene ID
- 7153
- UniProt ID
- P11388
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1812MZ | Recombinant Mouse Anti-Human TOP2A Antibody (clone Lj-T2) | IHC-P | Mouse antibody |
| ZG-0129C | Mouse Anti-TOP2A Recombinant Antibody (clone ABT-TOPO) | IHC-P, IF/ICC | Mouse IgG |
| ZG-0130C | Mouse Anti-TOP2A Recombinant Antibody (clone PT0247) | IHC-P, IF/ICC | Mouse IgG |
| VS3-CJ593 | Mouse Anti-TOP2A Recombinant Antibody (VS3-CJ593) | WB, IF, IHC | Mouse IgG2b |
| VS3-FY2905 | Mouse Anti-TOP2A Recombinant Antibody (VS3-FY2905) | WB, ICC, FC, ELISA | Mouse IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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