Rabbit Anti-TOP2A Recombinant Antibody (clone 5B10)
CAT#: ZG-0598U
Rabbit Anti-TOP2A Antibody (clone 5B10) displayed high specificity against the TOP2A protein of Human and Mouse.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-TOP2A Antibody (ZG-0598U) in WB
Western Blot
Positive WB detected in: Hela whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, NIH/3T3 whole cell lysate
All lanes: TOP2A antibody at 1:1500
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 175, 178, 179, 183 kDa
Observed band size: 175 kDa
IHC
Figure 2 Rabbit Anti-TOP2A Antibody (ZG-0598U) in IHC
IHC image of ZG-0598U diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6458/73_E8_2_selected.jpg
Normal Tissue
Figure 2 Testis
Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: Medium Intensity: Strong Quantity: <25% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2448/6156_A_5_6.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000131747-TOP2A
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Specifications
- Immunogen
- A synthesized peptide derived from human Topoisomerase II alpha
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TOP2A
- Species Reactivity
- Human, Mouse
- Clone
- 5B10
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Malignant Peripheral Nerve Sheath Tumor and Female Breast Cancer.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "TOP2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1962z | Mouse Anti-TOP2A Recombinant Antibody (clone 40D5) | WB, FC, IF, IHC | Mouse IgG2b, κ |
| MRO-1512-CN | Rabbit Anti-TOP2A Recombinant Antibody (clone CBACN-556) | WB, IHC, IP | Rabbit IgG |
| VS3-CJ593 | Mouse Anti-TOP2A Recombinant Antibody (VS3-CJ593) | WB, IF, IHC | Mouse IgG2b |
| VS3-CJ594 | Rabbit Anti-TOP2A Recombinant Antibody (VS3-CJ594) | WB, IHC, IP | Rabbit IgG |
| VS3-FY2905 | Mouse Anti-TOP2A Recombinant Antibody (VS3-FY2905) | WB, ICC, FC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3639 | Rabbit Anti-TOP2A Recombinant Antibody (clone DS3639AB) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-YF296 | A*02:01/Human Topoisomerase II-alpha-b (FLYDDNQRV) MHC Monomer | MHC Multimer |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7427 | Anti-TOP2A Immunohistochemistry Kit | IHC | |
| VS-0525-XY7428 | Anti-Mouse TOP2A Immunohistochemistry Kit | IHC |
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