Rabbit Anti-TPX2 Recombinant Antibody (clone R05-1B5)
CAT#: VS3-FY2095
The anti-TPX2 antibody detected in immunocytochemistry analysis of TPX2 in Hela. As a rabbit recombinant monoclonal antibody that demonstrates high specificity in binding to human TPX2. This R05-1B5 antibody is validated for immunocytochemistry analysis of TPX2 (green) in Hela and western blot analysis of TPX2 in Hela, A549, HL-60 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line HEL
Immunofluorescent staining of human cell line HEL shows localization to nucleoplasm, cytokinetic bridge & mitotic spindle.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5487/1825_A5_31_cr5ac33e6e0739c_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5487/15405_B_8_5.jpg
Normal Tissue
Figure 3 Lymph node
nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5487/15405_A_9_8.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000088325-TPX2
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ICC
Figure 1 Anti-TPX2 Antibody (VS3-FY2095) in ICC.
Immunocytochemistry staining of TPX2 (green) in Hela using the anti-TPX2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-TPX2 Antibody (VS3-FY2095) in WB.
The TPX2 expression in Hela, A549, HL-60 lysates were detected using the anti-TPX2 by western blot assay.
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Specifications
- Immunogen
- Recombinant human TPX2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TPX2
- Species Reactivity
- Human
- Clone
- R05-1B5
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 86 kDa, while the observed molecular weight stands at 100 kDa.
- Related Disease
- Uterine Corpus Endometrial Carcinoma and Hepatocellular Carcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- TPX2, Microtubule Nucleation Factor; Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2; Hepatocellular Carcinoma-Associated Antigen 519; Hepatocellular Carcinoma-Associated Antigen 90; Protein Fls353; C20orf1; C20orf2; HCA519; DIL-2; P100; DIL2; Restricted Expression Proliferation Associated Protein 100; Restricted Expression Proliferation-Associated Protein 100
- Gene ID
- 22974
- UniProt ID
- Q9ULW0
- Sequence Similarities
- Belongs to the TPX2 family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Microtubule, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys44, Lys48, Lys75, Lys524, and Lys567 (NX_Q9ULW0 [NX_Q9ULW0-1])
Modification sites at PhosphoSitePlus (Q9ULW0)
Glycosylation from GlyGen (Q9ULW0) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011526999.1; XP_011527001.1; XP_011527002.1; XP_047295973.1; XP_047295974.1
- Function
- Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TPX2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1163 | Mouse Anti-TPX2 Recombinant Antibody (clone 3H10-F8-D7) | WB | Mouse IgG1 |
| VS3-QX1164 | Mouse Anti-TPX2 Recombinant Antibody (clone 19AT738.292) | WB | Mouse IgG1 |
| VS3-WK1211 | Mouse Anti-TPX2 Recombinant Antibody (VS3-WK1211) | WB | Mouse IgG1 |
| VS3-WK1212 | Mouse Anti-TPX2 Recombinant Antibody (VS3-WK1212) | WB | Mouse IgG |
| VS3-FY1482 | Recombinant Rabbit Anti-TPX2 Antibody (clone R01-8A6) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2342 | Anti-TPX2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7483 | Anti-Mouse TPX2 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7482 | Anti-Human TPX2 Immunohistochemistry Kit | IHC |
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