Rabbit Anti-TPX2 Recombinant Antibody (clone R05-1B5) (CAT#: VS3-FY2095)
The anti-TPX2 antibody detected in immunocytochemistry analysis of TPX2 in Hela. As a rabbit recombinant monoclonal antibody that demonstrates high specificity in binding to human TPX2. This R05-1B5 antibody is validated for immunocytochemistry analysis of TPX2 (green) in Hela and western blot analysis of TPX2 in Hela, A549, HL-60 lysates.
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ICC
Figure 1 Anti-TPX2 Antibody (VS3-FY2095) in ICC.
Immunocytochemistry staining of TPX2 (green) in Hela using the anti-TPX2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
WB
Figure 2 Anti-TPX2 Antibody (VS3-FY2095) in WB.
The TPX2 expression in Hela, A549, HL-60 lysates were detected using the anti-TPX2 by western blot assay.
Specifications
- Immunogen
- Recombinant human TPX2 protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human TPX2
- Species Reactivity
- Human
- Clone
- R05-1B5
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 86 kDa, while the observed molecular weight stands at 100 kDa.
- Related Disease
- Uterine Corpus Endometrial Carcinoma and Hepatocellular Carcinoma
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- TPX2, Microtubule Nucleation Factor; Differentially Expressed In Cancerous And Non-Cancerous Lung Cells 2; Hepatocellular Carcinoma-Associated Antigen 519; Hepatocellular Carcinoma-Associated Antigen 90; Protein Fls353; C20orf1; C20orf2; HCA519; DIL-2; P100; DIL2; Restricted Expression Proliferation Associated Protein 100; Restricted Expression Proliferation-Associated Protein 100
- Gene ID
- 22974
- UniProt ID
- Q9ULW0
- Sequence Similarities
- Belongs to the TPX2 family.
- Cellular Localization
- Cytoplasm, Cytoskeleton, Microtubule, Nucleus
- Post Translation Modifications
- Ubiquitination at Lys44, Lys48, Lys75, Lys524, and Lys567 (NX_Q9ULW0 [NX_Q9ULW0-1])
Modification sites at PhosphoSitePlus (Q9ULW0)
Glycosylation from GlyGen (Q9ULW0) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011526999.1; XP_011527001.1; XP_011527002.1; XP_047295973.1; XP_047295974.1
- Function
- Activates AURKA by promoting its autophosphorylation at 'Thr-288' and protects this residue against dephosphorylation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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IgG Antibody Products
IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-QX1163 | Mouse Anti-TPX2 Recombinant Antibody (clone 3H10-F8-D7) | WB | Mouse IgG1 |
| VS3-QX1164 | Mouse Anti-TPX2 Recombinant Antibody (clone 19AT738.292) | WB | Mouse IgG1 |
| VS3-WK1211 | Mouse Anti-TPX2 Recombinant Antibody (VS3-WK1211) | WB | Mouse IgG1 |
| VS3-WK1212 | Mouse Anti-TPX2 Recombinant Antibody (VS3-WK1212) | WB | Mouse IgG |
| VS3-FY1482 | Recombinant Rabbit Anti-TPX2 Antibody (clone R01-8A6) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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