Rabbit Anti-TRAF4 Recombinant Antibody (clone R06-5G5) (CAT#: VS3-XY3529)
The rabbit recombinant antibody specifically recognizes TRAF4 (TNF receptor associated factor 4), which regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. TRAF4 also promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation. This antibody specifically reacts with human and mouse TRAF4. It has been tested using Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line RT4
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm, nucleoli fibrillar center & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21904/1943_G1_3_selected.jpg
(Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm, nucleoli fibrillar center & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/21904/1943_G1_3_selected.jpg
Figure 2 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9055/963_A12_1_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli fibrillar center.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/9055/963_A12_1_selected.jpg
Figure 3 Gallbladder
(High expression in gallbladder)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_4_4_val_selected.jpg
(High expression in gallbladder)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_4_4_val_selected.jpg
Figure 4 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_6_6.jpg
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_6_6.jpg
Figure 5 Salivary gland
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_B_3_1.jpg
(Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_B_3_1.jpg
Figure 6 Colon
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_7_3.jpg
(Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_7_3.jpg
Figure 7 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_9_4.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076604-TRAF4
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076604-TRAF4
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse TRAF4
- Species Reactivity
- Human, Mouse
- Clone
- R06-5G5
- Applications
- WB, ICC, IF, FC
- Related Disease
- Lung Cancer; Intraneural Perineurioma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- CART1; MLN62; RNF83
- Gene ID
- 9618
- UniProt ID
- Q9BUZ4
- Long Name
- TNF receptor associated factor 4
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Polyubiquitinated, leading to its proteasomal degradation (PubMed:19937093).
Ubiquitinated at Lys-263 by the SCF(FBXL2) complex, leading to its degradation by the proteasome.
- Protein Refseq
- XP_011523807.1
- Function
- Adapter protein with E3 ligase activity that is involved in many diverse biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation or apoptosis (PubMed:30352854, 31076633, 32268273, 33991522).
Promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation (PubMed:30352854).
Ubiquitinates SMURF2 through 'Lys-48'-linked ubiquitin chain leading to SMURF2 degradation through the proteasome and subsequently osteogenic differentiation.
Recommended Products
Isotype Control
Recommended Dilution Buffer
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0835MZ | Recombinant Mouse Anti-Human TRAF4 Antibody | IHC-P | Mouse antibody |
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