Rabbit Anti-TRAF4 Recombinant Antibody (clone R06-5G5)
CAT#: VS3-XY3529
The rabbit recombinant antibody specifically recognizes TRAF4 (TNF receptor associated factor 4), which regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. TRAF4 also promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation. This antibody specifically reacts with human and mouse TRAF4. It has been tested using Western Blot, Flow cytometry, Immunocytochemistry or Immunofluorescence.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RT4
Immunofluorescent staining of human cell line RT4 shows localization to nucleoplasm, nucleoli fibrillar center & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/21904/1943_G1_3_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli fibrillar center.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/9055/963_A12_1_selected.jpg
Normal Tissue
Figure 3 Gallbladder
High expression in gallbladder
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_4_4_val_selected.jpg
Normal Tissue
Figure 4 Testis
Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_6_6.jpg
Normal Tissue
Figure 5 Salivary gland
Glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_B_3_1.jpg
Normal Tissue
Figure 6 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52377/129872_A_9_4.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076604-TRAF4
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse TRAF4
- Species Reactivity
- Human, Mouse
- Clone
- R06-5G5
- Applications
- WB, ICC, IF, FC
- Related Disease
- Lung Cancer; Intraneural Perineurioma
Product Property
- Clonality
- Monoclonal
- Aggregation
- < 5%
- Format
- Liquid
- Buffer
- PBS (pH 7.4), 150 mM NaCl and 50% glycerol
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- WB: 1/500-1/1000
IF: 1/50-1/200
FC: 1/50-1/100
Target
- Alternative Names
- CART1; MLN62; RNF83
- Gene ID
- 9618
- UniProt ID
- Q9BUZ4
- Long Name
- TNF receptor associated factor 4
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Polyubiquitinated, leading to its proteasomal degradation (PubMed:19937093).
Ubiquitinated at Lys-263 by the SCF(FBXL2) complex, leading to its degradation by the proteasome.
- Protein Refseq
- XP_011523807.1
- Function
- Adapter protein with E3 ligase activity that is involved in many diverse biological processes including cell proliferation, migration, differentiation, DNA repair, platelet activation or apoptosis (PubMed:30352854, 31076633, 32268273, 33991522).
Promotes EGFR-mediated signaling by facilitating the dimerization of EGFR and downstream AKT activation thereby promoting cell proliferation (PubMed:30352854).
Ubiquitinates SMURF2 through 'Lys-48'-linked ubiquitin chain leading to SMURF2 degradation through the proteasome and subsequently osteogenic differentiation.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TRAF4"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0835MZ | Recombinant Mouse Anti-Human TRAF4 Antibody | IHC-P | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3670 | Rabbit Anti-TRAF4 Recombinant Antibody (clone DS3670AB) | FC, ICC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC384 | AbPlus™ Anti-TRAF4 Magnetic Beads (VS-0724-YC384) | IP, Protein Purification |
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