Rabbit Anti-TSC2 Recombinant Antibody (clone R04-1I3) (CAT#: VS3-FY2098)

• Immunogen
• Recombinant human potato protein protein

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human TSC2

• Species Reactivity
• Human

• Clone
• R04-1I3

• Applications
• Western Blot, Immunoprecipitation

• MW
• The calculated weight of the target protein is approximately 201 kDa, while the observed molecular weight stands at 201 kDa.

• Related Disease
• Lymphangioleiomyomatosis and Focal Cortical Dysplasia, Type Ii

• Purification
• Affinity Purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA

• Preservative
• 0.01% Sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/1000
  Immunoprecipitation: 1/20

• Alternative Names
• TSC Complex Subunit 2; Protein Phosphatase 1, Regulatory Subunit 160; Tuberous Sclerosis 2 Protein; TSC4; Tuberous Sclerosis 2; PPP1R160; Tuberin; LAM

• Gene ID
• 7249

• UniProt ID
• P49815

• Cellular Localization
• Cytoplasm, Membrane

• Post Translation Modifications
• Phosphorylation at Ser-1387, Ser-1418 or Ser-1420 does not affect interaction with TSC1. Phosphorylation at Ser-939 and Thr-1462 by PKB/AKT1 is induced by growth factor stimulation. Phosphorylation by AMPK activates it and leads to negative regulation of the mTORC1 complex. Phosphorylated at Ser-1798 by RPS6KA1; phosphorylation inhibits TSC2 ability to suppress mTORC1 signaling. Phosphorylated by DAPK1., Ubiquitinated by the DCX(FBXW5) E3 ubiquitin-protein ligase complex, leading to its subsequent degradation. Ubiquitinated by MYCBP2 independently of its phosphorylation status leading to subsequent degradation; association with TSC1 protects from ubiquitination.

• Protein Refseq
• NP_000539.2; NP_001070651.1; NP_001107854.1

• Function
• In complex with TSC1, this tumor suppressor inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling.
  Acts as a GTPase-activating protein (GAP) for the small GTPase RHEB, a direct activator of the protein kinase activity of mTORC1.
  May also play a role in microtubule-mediated protein transport (By similarity).
  Also stimulates the intrinsic GTPase activity of the Ras-related proteins RAP1A and RAB5 (By similarity).

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

Secondary Antibody

• 2ND-054CQ
• Goat Anti-Rabbit IgG Antibody, R-PE

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-055CQ
• Chicken Anti-Rabbit IgG (H&L) Antibody

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