Rabbit Anti-TTF2 Recombinant Antibody (clone R04-3F1) (CAT#: VS3-FY1919)

This R04-3F1 recombinant monoclonal antibody is specifically engineered to target human TTF2. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The transcription termination factor 2 antibody has been tested by western blot analysis of TTF2 in K562, rat brain, C6 lysates and immunocytochemistry analysis of TTF2 (green) in Hela using TTF2 antibody.


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WB

Figure 1 Anti-TTF2 Antibody (VS3-FY1919) in WB.

Figure 1 Anti-TTF2 Antibody (VS3-FY1919) in WB.

The TTF2 expression in K562, rat Brain, C6 lysates were detected using the anti-Transcription Termination Factor 2 by western blot assay.

ICC

Figure 2 Anti-TTF2 Antibody (VS3-FY1919) in ICC.

Figure 2 Anti-TTF2 Antibody (VS3-FY1919) in ICC.

Immunocytochemistry staining of TTF2 (green) in Hela using the anti-TTF2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).


Specifications

  • Immunogen
  • Synthetic peptide of human TTF2
  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human and Rat TTF2
  • Species Reactivity
  • Human, Rat
  • Clone
  • R04-3F1
  • Applications
  • Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
  • MW
  • The calculated weight of the target protein is approximately 130 kDa, while the observed molecular weight stands at 130 kDa.
  • Related Disease
  • Sexual Sadism and Narcissistic Personality Disorder

Product Property

  • Purification
  • Affinity Purified
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100
    Immunofluorescence: 1/50-1/200

Target

  • Alternative Names
  • Transcription Termination Factor 2; Transcription Release Factor 2; Transcription Termination Factor, RNA Polymerase II; RNA Polymerase II Termination Factor; Zinc Finger, GRF-Type Containing 6; Lodestar Homolog; HuF2
  • Sequence Similarities
  • Belongs to the SNF2/RAD54 helicase family.
  • Cellular Localization
  • Cytoplasm, Nucleus, Spliceosome
  • Post Translation Modifications
  • Ubiquitination at Lys567, Lys814, and Lys1109 (NX_Q9UNY4 [NX_Q9UNY4-1])
    Modification sites at PhosphoSitePlus (Q9UNY4)
    Glycosylation from GlyGen (Q9UNY4) 1 site, 1 O-linked glycan (1 site)
  • Function
  • DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.

Recommended Products

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

Download resources about recombinant antibody development and antibody engineering to boost your research.

See other products for "TTF2"

Recombinant Antibody

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-3716 Rabbit Anti-TTF2 Recombinant Antibody (clone DS3716AB) ICC, IF, IHC-P, WB, FC Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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