Rabbit Anti-TTF2 Recombinant Antibody (clone R04-3F1) (CAT#: VS3-FY1919)
This R04-3F1 recombinant monoclonal antibody is specifically engineered to target human TTF2. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The transcription termination factor 2 antibody has been tested by western blot analysis of TTF2 in K562, rat brain, C6 lysates and immunocytochemistry analysis of TTF2 (green) in Hela using TTF2 antibody.
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WB
Figure 1 Anti-TTF2 Antibody (VS3-FY1919) in WB.
The TTF2 expression in K562, rat Brain, C6 lysates were detected using the anti-Transcription Termination Factor 2 by western blot assay.
ICC
Figure 2 Anti-TTF2 Antibody (VS3-FY1919) in ICC.
Immunocytochemistry staining of TTF2 (green) in Hela using the anti-TTF2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
Specifications
- Immunogen
- Synthetic peptide of human TTF2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat TTF2
- Species Reactivity
- Human, Rat
- Clone
- R04-3F1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 130 kDa, while the observed molecular weight stands at 130 kDa.
- Related Disease
- Sexual Sadism and Narcissistic Personality Disorder
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Transcription Termination Factor 2; Transcription Release Factor 2; Transcription Termination Factor, RNA Polymerase II; RNA Polymerase II Termination Factor; Zinc Finger, GRF-Type Containing 6; Lodestar Homolog; HuF2
- Gene ID
- 8458
- UniProt ID
- Q9UNY4
- Sequence Similarities
- Belongs to the SNF2/RAD54 helicase family.
- Cellular Localization
- Cytoplasm, Nucleus, Spliceosome
- Post Translation Modifications
- Ubiquitination at Lys567, Lys814, and Lys1109 (NX_Q9UNY4 [NX_Q9UNY4-1])
Modification sites at PhosphoSitePlus (Q9UNY4)
Glycosylation from GlyGen (Q9UNY4) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016858039.1; XP_016858040.1; XP_016858041.1; XP_016858044.1; XP_047288091.1
- Function
- DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2055CT | Recombinant Mouse anti-Human TTF2 Monoclonal antibody (EML884) | WB |
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