Rabbit Anti-TTF2 Recombinant Antibody (clone R04-3F1)
CAT#: VS3-FY1919
This R04-3F1 recombinant monoclonal antibody is specifically engineered to target human TTF2. It cross-reacts with rat. It is suitable for use in a variety of assays, including WB, IHC-F, IHC-P, ICC/IF. Applications Tested: The transcription termination factor 2 antibody has been tested by western blot analysis of TTF2 in K562, rat brain, C6 lysates and immunocytochemistry analysis of TTF2 (green) in Hela using TTF2 antibody.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70762/1354_H2_1_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5776/20558_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5776/20558_A_9_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5776/20558_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5776/20558_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5776/20558_A_4_6.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5776/20558_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000116830-TTF2
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WB
Figure 1 Anti-TTF2 Antibody (VS3-FY1919) in WB.
The TTF2 expression in K562, rat Brain, C6 lysates were detected using the anti-Transcription Termination Factor 2 by western blot assay.
ICC
Figure 2 Anti-TTF2 Antibody (VS3-FY1919) in ICC.
Immunocytochemistry staining of TTF2 (green) in Hela using the anti-TTF2 antibody. Nuclear DNA was labeled with DAPI (shown in blue).
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Specifications
- Immunogen
- Synthetic peptide of human TTF2
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat TTF2
- Species Reactivity
- Human, Rat
- Clone
- R04-3F1
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 130 kDa, while the observed molecular weight stands at 130 kDa.
- Related Disease
- Sexual Sadism and Narcissistic Personality Disorder
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Transcription Termination Factor 2; Transcription Release Factor 2; Transcription Termination Factor, RNA Polymerase II; RNA Polymerase II Termination Factor; Zinc Finger, GRF-Type Containing 6; Lodestar Homolog; HuF2
- Gene ID
- 8458
- UniProt ID
- Q9UNY4
- Sequence Similarities
- Belongs to the SNF2/RAD54 helicase family.
- Cellular Localization
- Cytoplasm, Nucleus, Spliceosome
- Post Translation Modifications
- Ubiquitination at Lys567, Lys814, and Lys1109 (NX_Q9UNY4 [NX_Q9UNY4-1])
Modification sites at PhosphoSitePlus (Q9UNY4)
Glycosylation from GlyGen (Q9UNY4) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_016858039.1; XP_016858040.1; XP_016858041.1; XP_016858044.1; XP_047288091.1
- Function
- DsDNA-dependent ATPase which acts as a transcription termination factor by coupling ATP hydrolysis with removal of RNA polymerase II from the DNA template. May contribute to mitotic transcription repression. May also be involved in pre-mRNA splicing.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TTF2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2055CT | Recombinant Mouse anti-Human TTF2 Monoclonal antibody (EML884) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3716 | Rabbit Anti-TTF2 Recombinant Antibody (clone DS3716AB) | ICC, IF, IHC-P, WB, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC71 | AbPlus™ Anti-TTF2 Magnetic Beads (VS-0724-YC71) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2386 | Anti-TTF2 Immunohistochemistry Kit | IHC |
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