Rabbit Anti-TUB Recombinant Antibody (clone R57-1T-4)
CAT#: VS3-FY2153
Clone R57-1T-4 is a rabbit recombinant monoclonal antibody that is specifically engineered to target human TUB protein, a calculated weight of 56 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R57-1T-4 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli & centriolar satellites.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17997/140_F10_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skin
Immunohistochemical staining of human skin shows strong cytoplasmic positivity in basal call layer of epidermis.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49019/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49019/146021_B_7_5.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49019/146021_A_8_4.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/49019/146021_A_4_6.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HAP1, HEK93, HeLa, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000166402-TUB
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat TUB
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R57-1T-4
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 56 kDa, while the observed molecular weight stands at 60kDa.
- Related Disease
- Retinal Dystrophy And Obesity and Retinitis Pigmentosa
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Tubby Bipartite Transcription Factor; Tubby (Mouse) Homolog; Tubby Homolog (Mouse); Tubby Protein Homolog; Tubby Homologue; Tubby Homolog; RDOB; Rd5
- Gene ID
- 7275
- UniProt ID
- P50607
- Sequence Similarities
- Belongs to the TUB family.
- Cellular Localization
- Cell membrane, Cytoplasm, Membrane, Nucleus, Secreted
- Post Translation Modifications
- Modification sites at PhosphoSitePlus (P50607)
Modification sites at neXtProt (NX_P50607)
Glycosylation from GlyGen (P50607) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_005253166.1; XP_011518646.1; XP_047283468.1; XP_047283469.1; XP_047283470.1
- Function
- Functions in signal transduction from heterotrimeric G protein-coupled receptors. Binds to membranes containing phosphatidylinositol 4,5-bisphosphate. Can bind DNA (in vitro). May contribute to the regulation of transcription in the nucleus. Could be involved in the hypothalamic regulation of body weight (By similarity).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TUB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3718 | Rabbit Anti-TUB Recombinant Antibody (clone DS3718AB) | WB, IHC, ICC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7598 | Anti-TUB Immunohistochemistry Kit | IHC |
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