Mouse Anti-TUBA4A Recombinant Antibody (clone 1C4E12)

CAT#: VS7-0425-WR1065

This product is a mouse IgG2a antibody that binds to human TUBA4A. The antibody 1C4E12 recognizes specific amino acid domain of human TUBA4A (AA: (299-447)). It can be used for WB, IHC, and FC assays.

Gene Expression
Figure 1 IF staining of human cell line A-431 Figure 2 IF staining of human cell line U-251 MG Figure 3 IF staining of human cell line U-2 OS Figure 4 IHC staining of human small intestine Figure 5 Lymph node Figure 6 Colon Figure 7 Pancreas Figure 8 Testis Figure 9 RNA cell line category: Cell line enhanced (HaCaT, HBEC3-KT)

Specifications

  • Immunogen
  • Recombinant human TUBA4A (AA: (299-447)) fragment expressed in E. Coli.
  • Host Species
  • Mouse
  • Type
  • Mouse IgG2a
  • Specificity
  • Human TUBA4A
  • Species Reactivity
  • Human
  • Clone
  • 1C4E12
  • Molecule Class
  • Alpha-Tubulin Protein
  • Applications
  • WB, IHC, FC
  • Conjugate
  • Unconjugated
  • Related Disease
  • Amyotrophic lateral sclerosis 22, with or without frontotemporal dementia (ALS22)

Product Property

  • Clonality
  • Monoclonal
  • Purity
  • >95% as determined by SDS-PAGE and SEC-HPLC analysis
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Buffer
  • PBS
  • Preservative
  • 0.05% sodium azide
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • Western Blot: 1:500 - 1:2000
    Immunohistochemistry-Paraffin: 1:200 - 1:1000
    Flow Cytometry: 1:200 - 1:400
    Enzyme-linked Immunosorbent Assay: 1:10000

Target

  • Alternative Names
  • ALS22; TUBA1; H2-ALPHA
  • Long Name
  • Tubulin Alpha 4A
  • Cellular Localization
  • Cytoplasm; Cytoskeleton; Microtubule
  • Post Translation Modifications
  • Acetylation; Methylation; Nitration; Phosphoprotein
  • Function
  • Hydrolase
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

MSDS

COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

Reconstitution Buffer

Recommended Dilution Buffer

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