Mouse Anti-TUBB Recombinant Antibody (VS3-CJ470) (CAT#: VS3-CJ470)

• Immunogen
• Recombinant protein of TBB5

• Host Species
• Mouse

• Type
• Mouse IgG2b, κ

• Specificity
• Human, Mouse, Rat TUBB

• Species Reactivity
• Human, Mouse, Rat

• Applications
• WB, IF, IHC-P

• Conjugate
• Unconjugated

• Purification
• Protein G affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Format
• Liquid

• Buffer
• PBS, pH 7.3.

• Preservative
• 0.09% Sodium Azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• This antibody has been tested for use in Western Blot (1:1000), Immunofluorescence (1:25), Immunohistochemistry-Parraffin (1:25).

• Alternative Names
• M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56

• Gene ID
• 203068

• UniProt ID
• P07437

• Sequence Similarities
• Belongs to the tubulin family.

• Cellular Localization
• Cytoplasm, Cytoskeleton, Microtubule

• Post Translation Modifications
• Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866, PubMed:28576883).
  Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).
  Glutamylation is also involved in cilia motility (By similarity).
  Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into cilia and flagella axonemes, which is required for their stability and maintenance. Flagella glycylation controls sperm motility (Probable) (PubMed:28576883).
  Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally (Probable) (PubMed:28576883).
  Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

• Protein Refseq
• NP_001280141.1; NP_001280142.1; NP_001280143.1

• Function
• Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Isotype Control

• MOB-212YJL
• Mouse IgG2b kappa Isotype Control Antibody (Mopc21)

• MOB-203CQ
• Mouse IgG2b, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS