Mouse Anti-TUBB1 Recombinant Antibody (ZG-0522F)
CAT#: ZG-0522F
This product is a mouse antibody that recognizes TUBB1.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-TUBB1 Recombinant Antibody (ZG-0522F) in WB.
Tubulin beta mouse mAb (1:5000 dilution) was used to detect Tubulin beta in COS7, LNCap, MCF7, and C2C12 cell lysates by Western blot. Predicted band size: 55KDa. Observed band size: 55KDa.
ICC
Figure 2 Mouse Anti-TUBB1 Recombinant Antibody (ZG-0522F) in ICC.
Immunocytochemical staining was performed on HeLa cells fixed with 4% paraformaldehyde, and anti-tubulin beta mouse mAb (diluted 1:500) was used.
WB
Figure 3 Mouse Anti-TUBB1 Recombinant Antibody (ZG-0522F) in WB.
Tubulin beta mouse mAb (diluted 1:5000-1:10000) was used to detect Tubulin beta in goat intestine, goat skeletal muscle, goat spleen, and goat lung cell lysates by western blot. Predicted band size: 55KDa. Observed band size: 55KDa.
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Normal Tissue
Figure 1 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4286/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_7_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_8_5.jpg
Normal Tissue
Figure 5 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75% Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Round or early spermatids Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Medium Intensity: Moderate Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_6_6.jpg
Normal Tissue
Figure 6 Fallopian tube
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_7_7.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/46280/103953_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enriched (HEL)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101162-TUBB1
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Specifications
- Immunogen
- Recombinant human Tubulin beta protein fragments expressed in
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TUBB1
- Species Reactivity
- Human, Mouse, Monkey, Goat, Hamster, Rat, chlamydomonas
- Applications
- Western Blot, Immunocytochemistry
- Conjugate
- NONE
Product Property
- Format
- Liquid
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:5000
Immunocytochemistry: 1:500
Target
- Alternative Names
- Tubulin Beta 1 Class VI; Class VI Beta-Tubulin; Tubulin, Beta 1; beta 4 tubulin; Beta 5 tubulin; beta Ib tubulin; M40; TUBB1; TUBB5; tubulin beta 1 chain; Tubulin beta 4 chain; tubulin beta 5 chain; Tubulin beta; Tubulin beta chain; tubulin beta polypeptide.
- Gene ID
- 81027
- UniProt ID
- Q9H4B7
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1382 | PE-B-FIV21/Gallus gallus TUBB1 (REVDEQLLSV) MHC Tetramer | FCM | |
| MHC-LC1383 | APC-B-FIV21/Gallus gallus TUBB1 (REVDEQLLSV) MHC Tetramer | FCM | |
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0521F | Mouse Anti-TUBB1 Recombinant Antibody (ZG-0521F) | WB | Mouse IgG |
| ZG-0200C | Mouse Anti-TUBB1 Recombinant Antibody (ZG-0200C) | WB | Mouse IgG |
| ZG-0201C | Mouse Anti-TUBB1 Recombinant Antibody (clone 3F7) | WB, IHC-P, IF | Mouse IgG |
| ZG-161R | Mouse Anti-TUBB1 Recombinant Antibody (ZG-161R) | WB | Mouse IgG |
| VS7-0425-WR1068 | Mouse Anti-TUBB1 Recombinant Antibody (clone 4D4G6) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY521 | Mouse Anti-NHP TUBB1 Recombinant Antibody (clone 2A1A9) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7616 | Anti-TUBB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7617 | Anti-Mouse TUBB1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7618 | Anti-Bovine TUBB1 Immunohistochemistry Kit | IHC |
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