Mouse Anti-TUBB3 Monoclonal Antibody (VS7-0425-WR1071)
CAT#: VS7-0425-WR1071
This product is a mouse IgG antibody that binds to human TUBB3. The antibody can be used for WB, IF, ICC, FC, and ELISA assays.
Gene Expression
RNA Expression
Figure 1 RNA cell line category: Cell line enhanced (A549, SCLC-21H, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000258947-TUBB3
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human TUBB3
- Species Reactivity
- Human
- Molecule Class
- Beta Tubulin Protein
- Applications
- WB, IF, ICC, FC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- Fibrosis of extraocular muscles, congenital, 3A (CFEOM3A); Cortical dysplasia, complex, with other brain malformations 1 (CDCBM1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- TUBB3; TUBB4; Class III Beta-Tubulin; Tubulin Beta-3 Chain; FEOM3; CFEOM3A; BETA-4; Tubulin, Beta 3; Tubulin Beta-III
- Gene ID
- 10381
- UniProt ID
- Q13509
- Long Name
- Tubulin Beta 3 Class III
- Cellular Localization
- Cell projection; Cytoplasm; Cytoskeleton; Microtubule
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein
- Protein Refseq
- NP_001184110.1
- Function
- Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871).; Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871).; Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871).; TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521).; Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977).; Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TUBB3"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2752z | Mouse Anti-TUBB3 Recombinant Antibody (clone 21A5) | WB, ELISA, FC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PABL-411 | Human Anti-TUBB3 Recombinant Antibody (clone YC093) | WB, ELISA, FuncS | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PFBL-408 | Human Anti-TUBB3 Recombinant Antibody (clone YC094); Fab Fragment | WB, ELISA, FuncS | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| PSBL-408 | Human Anti-TUBB3 Recombinant Antibody (clone YC094); scFv Fragment | WB, ELISA, FuncS | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0539MZ | Recombinant Mouse Anti-Human TUBB3 Antibody (clone UV-30) | IHC-P | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0739MZ | Chicken Anti-TUBB3 Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3723 | Rabbit Anti-TUBB3 Recombinant Antibody (clone DS3723AB) | WB, FC, IP, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-109CQ | Mouse Anti-TUBB3 Recombinant Antibody (clone YC153) | ELISA, WB, IHC, ICC, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1079-CN | Mouse Anti-TUBB3 Recombinant Antibody (clone A8-D10) | WB, IF, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0072-FY | Rabbit Anti-TUBB3 Recombinant Antibody (clone AFY0043) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1458 | AbPlus™ Anti-TUBB3 Magnetic Beads (VS-0724-YC1458) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2394 | Anti-TUBB3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7625 | Anti-Mouse TUBB3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7624 | Anti-Human TUBB3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7626 | Anti-Monkey TUBB3 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7627 | Anti-Rat TUBB3 Immunohistochemistry Kit | IHC |
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