CytoStream™ Rabbit Anti-UBA1 Recombinant Antibody (VS13-YC1199)
CAT#: VS13-YC1199
The CytoStream™ Rabbit Anti-UBA1 Recombinant Antibody is specific for human and mouse UBA1. UBA1 catalyzes the initial step of ubiquitin conjugation for protein degradation and may function in DNA repair. It's located on chromosome Xp11.23. The anti-UBA1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1506/25_G6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/618_D7_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73410/153526_B_7_8_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_5_6.jpg
Normal Tissue
Figure 5 Skin
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_B_7_1.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_8.jpg
Normal Tissue
Figure 7 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_B_8_5.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_5.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130985-UBA1
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Specifications
- Immunogen
- Recombinant human UBA1, aa 1009-1058.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse UBA1
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Ubiquitin Like Modifier Activating Enzyme 1; Ubiquitin-Activating Enzyme E1; A1S9T; UBE1; Ubiquitin-Activating Enzyme E1 (A1S9T And BN75 Temperature Sensitivity Complementing); UBA1, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); A1S9T And BN75 Temperature Sensitivity Complementing; POC20 Centriolar Protein Homolog (Chlamydomonas); UBA1, Ubiquitin-Activating Enzyme E1 Homolog A; Ubiquitin-Like Modifier Activating Enzyme 1; Ubiquitin-Like Modifier-Activating Enzyme 1; Testicular Secretory Protein Li 63; POC20 Centriolar Protein Homolog;
- Gene ID
- 7317
- UniProt ID
- P22314
- Long Name
- Ubiquitin Like Modifier Activating Enzyme 1
- Cellular Localization
- Nucleus; Mitochondrion; Cytoplasm
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "UBA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3859z | Mouse Anti-UBA1 Recombinant Antibody (clone 40G1) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| MRO-1553-CN | Rabbit Anti-UBA1 Recombinant Antibody (clone CBACN-572) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-CJ786 | Rabbit Anti-UBA1 Recombinant Antibody (VS3-CJ786) | WB, ICC, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3741 | Rabbit Anti-UBA1 Recombinant Antibody (clone DS3741AB) | WB, FC, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1520 | AbPlus™ Anti-UBA1 Magnetic Beads (VS-0724-YC1520) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2410 | Anti-UBA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7678 | Anti-Human UBA1 Immunohistochemistry Kit | IHC |
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