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    CytoStream™ Rabbit Anti-UBA1 Recombinant Antibody (VS13-YC1199) (CAT#: VS13-YC1199)

    The CytoStream™ Rabbit Anti-UBA1 Recombinant Antibody is specific for human and mouse UBA1. UBA1 catalyzes the initial step of ubiquitin conjugation for protein degradation and may function in DNA repair. It's located on chromosome Xp11.23. The anti-UBA1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).

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    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
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    Purity :
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    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-251 MG
    Figure 1 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/1506/25_G6_1_selected.jpg
    Figure 2 IF staining of human cell line A-431
    Figure 2 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/618_D7_2_selected.jpg
    Normal Tissue
    Figure 3 IHC staining of human cerebellum
    Figure 3 IHC staining of human cerebellum
    (Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/73410/153526_B_7_8_selected.jpg
    Figure 4 Testis
    Figure 4 Testis
    (Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_5_6.jpg
    Figure 5 Skin
    Figure 5 Skin
    (Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_B_7_1.jpg
    Figure 6 Lymph node
    Figure 6 Lymph node
    (Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_8.jpg
    Figure 7 Cerebral cortex
    Figure 7 Cerebral cortex
    (Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_B_8_5.jpg
    Figure 8 Colon
    Figure 8 Colon
    (Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_3.jpg
    Figure 9 Liver
    Figure 9 Liver
    (Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_4.jpg
    Figure 10 Kidney
    Figure 10 Kidney
    (Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_5.jpg
    RNA Expression
    Figure 11 RNA cell line category: Low cell line specificity
    Figure 11 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130985-UBA1

    Specifications

    • Immunogen
    • Recombinant human UBA1, aa 1009-1058.
    • Host Species
    • Rabbit
    • Type
    • Rabbit IgG
    • Specificity
    • Human and Mouse UBA1
    • Species Reactivity
    • Human, Mouse
    • Applications
    • WB, ICC, IF, IHC-P, FC
    • Conjugate
    • NONE

    Product Property

    • Clonality
    • Monoclonal
    • Purification
    • Protein A purified
    • Format
    • Liquid
    • Concentration
    • Please refer to the vial label for the specific concentration.
    • Buffer
    • TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
    • Preservative
    • 0.05% Sodium azide
    • Storage
    • Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Shipping with ice pack.

    Applications

    • Application Notes
    • Western blot (WB): 1/500-1/2000
      Immunocytochemistry (ICC): 1/50-1/200
      Immunofluorescence (IF): 1/50-1/200
      Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
      Flow cytometry (FC): 1/50-1/100

    Target

    • Alternative Names
    • Ubiquitin Like Modifier Activating Enzyme 1; Ubiquitin-Activating Enzyme E1; A1S9T; UBE1; Ubiquitin-Activating Enzyme E1 (A1S9T And BN75 Temperature Sensitivity Complementing); UBA1, Ubiquitin-Activating Enzyme E1 Homolog (Yeast); A1S9T And BN75 Temperature Sensitivity Complementing; POC20 Centriolar Protein Homolog (Chlamydomonas); UBA1, Ubiquitin-Activating Enzyme E1 Homolog A; Ubiquitin-Like Modifier Activating Enzyme 1; Ubiquitin-Like Modifier-Activating Enzyme 1; Testicular Secretory Protein Li 63; POC20 Centriolar Protein Homolog;
    • Long Name
    • Ubiquitin Like Modifier Activating Enzyme 1
    • Cellular Localization
    • Nucleus; Mitochondrion; Cytoplasm

    Recommended Products

    Secondary Antibody

    Isotype Control

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "UBA1"

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    CAT Product Name Application Type
    MOB-3859z Mouse Anti-UBA1 Recombinant Antibody (clone 40G1) WB, IP, IF, ELISA Mouse IgG2a, κ
    MRO-1553-CN Rabbit Anti-UBA1 Recombinant Antibody (clone CBACN-572) WB, IF, IHC, FC Rabbit IgG
    VS3-CJ786 Rabbit Anti-UBA1 Recombinant Antibody (VS3-CJ786) WB, ICC, IF, IHC, FC Rabbit IgG

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