Rabbit Anti-UBA1 Recombinant Antibody (VS3-CJ786)
CAT#: VS3-CJ786
This product is a rabbit antibody that recognizes human, mouse, and rat UBA1.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1506/25_G6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/618_D7_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/73410/153526_B_7_8_selected.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Leydig cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_5_6.jpg
Normal Tissue
Figure 5 Skin
Fibroblasts Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Keratinocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Langerhans Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Melanocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_B_7_1.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_8.jpg
Normal Tissue
Figure 7 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: nuclear Neuronal cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_B_8_5.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19435/43780_A_7_5.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130985-UBA1
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat UBA1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; VEXAS; CFAP124
- Gene ID
- 7317
- UniProt ID
- P22314
- Sequence Similarities
- Belongs to the ubiquitin-activating E1 family.
- Cellular Localization
- Cytoplasm, Mitochondrion, Nucleus
- Post Translation Modifications
- ISGylated.
- Protein Refseq
- NP_003325.2; NP_695012.1
- Function
- Catalyzes the first step in ubiquitin conjugation to mark cellular proteins for degradation through the ubiquitin-proteasome system (PubMed:1606621, PubMed:1447181, PubMed:33108101).
Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP (PubMed:1447181).
Essential for the formation of radiation-induced foci, timely DNA repair and for response to replication stress. Promotes the recruitment of TP53BP1 and BRCA1 at DNA damage sites (PubMed:22456334).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "UBA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3859z | Mouse Anti-UBA1 Recombinant Antibody (clone 40G1) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| MRO-1553-CN | Rabbit Anti-UBA1 Recombinant Antibody (clone CBACN-572) | WB, IF, IHC, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3741 | Rabbit Anti-UBA1 Recombinant Antibody (clone DS3741AB) | WB, FC, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1520 | AbPlus™ Anti-UBA1 Magnetic Beads (VS-0724-YC1520) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1199 | CytoStream™ Rabbit Anti-UBA1 Recombinant Antibody (VS13-YC1199) | WB, ICC, IF, IHC-P, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2410 | Anti-UBA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7678 | Anti-Human UBA1 Immunohistochemistry Kit | IHC |
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