Anti-UBE2A Antibody Prodrug, Protease Activated (AA2)
CAT#: VS-1025-YC161
The Anti-UBE2A Antibody Prodrug, Protease Activated (AA2) is an antibody prodrug comprised of a recombinant human antibody targeting human UBE2A, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to UBE2A protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to UBE2A effectively. UBE2A is an E2 ubiquitin-conjugating enzyme that is an oncogene when fused to the TFG gene, forming the TFG-UBE2A fusion found in a subset of myeloid malignancies. This fusion protein promotes oncogenesis through aberrant ubiquitination.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51765/769_F12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65898/1247_B7_3_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077721-UBE2A
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Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human UBE2A
- Target
- UBE2A
- Species Reactivity
- Human
- Isotype
- IgG
- Clone
- AA2
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants.
- Alternative Names
- UBC2; HHR6A; MRXSN; RAD6A; MRXS30
- Gene ID
- 7319
- UniProt ID
- P49459
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Datasheet
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Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "Clone AA2"
- CAT
- Product Name
See other products for "UBE2A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270YC | Human Anti-UBE2A Recombinant Antibody (clone AA2) | IF, IP, WB | Human IgG |
| VS3-FY2137 | Rabbit Anti-UBE2A Recombinant Antibody (clone R87-4N-5) | WB, IHC-P, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270-YC-S(P) | Human Anti-UBE2A Recombinant Antibody (clone AA2); scFv Fragment | IF, IP, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270-YC-F(E) | Human Anti-UBE2A Recombinant Antibody (clone AA2); Fab Fragment | IF, IP, WB | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY269 | AbPlus™ Anti-UBE2A Magnetic Beads (AA2) | IP, Protein Purification |
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