AbPlus™ Anti-UBE2A Magnetic Beads (AA2)
CAT#: VS-0424-XY269
The AbPlus Anti-UBE2A Magnetic Beads (AA2) is an innovative affinity resin which is bound with anti-UBE2A specific antibody (AA2). The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UBE2A protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & vesicles.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/51765/769_F12_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & plasma membrane.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/65898/1247_B7_3_selected.jpg
RNA Expression
Figure 3 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000077721-UBE2A
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magrose bead (> 50 μmol/mL gel)
- Bead Ligand
- Anti-UBE2A specific antibody (AA2)
- Target
- UBE2A
- Immunogen
- The Golgi-localized small GTPase UBE2A
- Target Species
- Human
- Antibody Clone
- AA2
- Bead Capacity
- 20-30 mg/mL binding antibody
- Bead size
- 10-37 μm
- Stability
- pH 2-14
- Format
- 20% Suspension
- Buffer
- PBS, pH 7.4, with 1% BSA
- Preservative
- 0.03% Proclin 300
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, ubiquitin-conjugating enzymes, and ubiquitin-protein ligases. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. This enzyme is required for post-replicative DNA damage repair, and may play a role in transcriptional regulation. Mutations in this gene are associated with cognitive disability. Alternative splicing results in multiple transcript variants.
- Alternative Names
- UBC2; HHR6A; MRXSN; RAD6A; MRXS30
- Gene ID
- 7319
- UniProt ID
- P49459
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270YC | Human Anti-UBE2A Recombinant Antibody (clone AA2) | IF, IP, WB | Human IgG |
| VS3-FY2137 | Rabbit Anti-UBE2A Recombinant Antibody (clone R87-4N-5) | WB, IHC-P, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270-YC-S(P) | Human Anti-UBE2A Recombinant Antibody (clone AA2); scFv Fragment | IF, IP, WB | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| FAMAB-0270-YC-F(E) | Human Anti-UBE2A Recombinant Antibody (clone AA2); Fab Fragment | IF, IP, WB | Human Fab |
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