Anti-UBE2C Immunohistochemistry Kit
CAT#: VS-0325-XY2415
Designed for cell cycle and cancer research, the UBE2C IHC Kit provides a complete reagent panel to detect this E2 ubiquitin conjugase in tumor tissues. Its protocol is optimized for paraffin-embedded FFPE and frozen biopsy sections, minimizing variability in mitotic index analysis. The pre-formulated reagents ensure reliable staining, ideal for assessing UBE2C's role in cell proliferation and chemoresistance.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34569/1216_F6_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/265_D2_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human stomach
Immunohistochemical staining of human stomach shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54975/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong cytoplasmic and nuclear positivity in purkinje cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11464/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_8.jpg
Normal Tissue
Figure 7 Tonsil
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Squamous epithelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_6_8.jpg
Normal Tissue
Figure 8 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_9_3.jpg
Normal Tissue
Figure 9 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_4.jpg
Normal Tissue
Figure 10 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/35990/71862_A_7_5.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000175063-UBE2C
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- UBE2C
- Primary Antibody
- Rabbit Anti-UBE2C Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Datasheet
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "UBE2C"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3665z | Mouse Anti-UBE2C Recombinant Antibody (clone 16G12) | WB, IHC, IF, ELISA | Mouse IgG1 |
| FAMAB-1285CQ | Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41C-6) | Immuno-MRM, WB | Mouse IgG1 |
| FAMAB-1286CQ | Mouse Anti-UBE2C Recombinant Antibody (clone SAIC-41A-1) | Immuno-MRM, WB, ELISA | Mouse IgG1 |
| VS3-XY1575 | Mouse Anti-UBE2C Recombinant Antibody (clone 1F5D3) | ELISA, WB, IHC | Mouse IgG2a |
| VS3-XY1576 | Mouse Anti-UBE2C Recombinant Antibody (clone 4C10A7) | ELISA, WB, IHC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3749 | Rabbit Anti-UBE2C Recombinant Antibody (clone DS3749AB) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-CN0057 | APC-B*15:02/Human Ubiquitin-conjugating enzyme E2C (ELWKNPTAF) MHC Tetramer | FCM | |
| MHC-CN0058 | PE-B*15:02/Human Ubiquitin-conjugating enzyme E2C (ELWKNPTAF) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY524 | Mouse Anti-NHP UBE2C Recombinant Antibody (clone 1F5D3) | WB, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7691 | Anti-Rat UBE2C Immunohistochemistry Kit | IHC | |
| VS-0525-XY7690 | Anti-Human UBE2C Immunohistochemistry Kit | IHC |
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