AbPlus™ Anti-UBE2M Magnetic Beads (VS-0724-YC804) (CAT#: VS-0724-YC804)
The AbPlus Anti-UBE2M Magnetic Beads (VS-0724-YC804) is an innovative affinity resin which is bound with anti-UBE2M specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UBE2M protein under native and denaturing conditions.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression

Figure 1 IF staining of human cell line HEK 293
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, nuclear bodies & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/1272_D2_4_selected.jpg
(Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, nuclear bodies & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/1272_D2_4_selected.jpg

Figure 2 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57800/1147_E12_3_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57800/1147_E12_3_selected.jpg

Figure 3 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows distinct cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57800/ihc_selected.jpg
(Immunohistochemical staining of human rectum shows distinct cytoplasmic and nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/57800/ihc_selected.jpg

Figure 4 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows strong nuclear and moderate cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4993/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows strong nuclear and moderate cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/4993/ihc_selected.jpg

Figure 5 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/137512_A_6_6.jpg
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/137512_A_6_6.jpg

Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/137512_A_9_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/137512_A_9_3.jpg

Figure 7 RNA cell line category: Cell line enhanced (HEK93)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130725-UBE2M
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130725-UBE2M
Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-UBE2M specific antibody
- Target
- UBE2M
- Immunogen
- Synthetic peptide corresponding to the C-terminus of the human UBE2M.
- Target Species
- Human, Mouse
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 M; Ubiquitin-Conjugating Enzyme E2M (UBC12 Homolog, Yeast); NEDD8 Carrier Protein; NEDD8 Protein Ligase; UBC12; Ubiquitin-Conjugating Enzyme E2M (Homologous To Yeast UBC12); Ubiquitin-Conjugating Enzyme E2 M; Ubiquitin-Conjugating Enzyme E2M; Ubiquitin Conjugating Enzyme E2M;
- Gene ID
- 9040
- UniProt ID
- P61081
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Non-Human Primate (NHP) Antibody Products
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-2123CT | Recombinant Mouse anti-Human UBE2M Monoclonal antibody (EML822) | WB |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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