AbPlus™ Anti-UBE2M Magnetic Beads (VS-0724-YC803)
CAT#: VS-0724-YC803
The AbPlus Anti-UBE2M Magnetic Beads (VS-0724-YC803) is an innovative affinity resin which is bound with anti-UBE2M specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of UBE2M protein under native and denaturing conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/1272_D2_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57800/1147_E12_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows distinct cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57800/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear and moderate cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4993/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/137512_A_6_6.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/137512_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130725-UBE2M
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Specifications
- Applications
- Immunoprecipitation, Protein Purification
- Matrix
- Magnetic bead
- Bead Ligand
- Anti-UBE2M specific antibody
- Target
- UBE2M
- Immunogen
- Recombinant human UBE2M, aa 1-183.
- Target Species
- Human
- Bead Capacity
- 40 mg/mL
- Bead size
- 25 μm
- Format
- Suspension
- Concentration
- 2 mg/mL
- Buffer
- PBS, pH 7.4
- Preservative
- 0.1% Sodium azide
- Storage
- Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.
Applications
- Application Notes
- The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.
Target
- Introduction
- The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein is linked with a ubiquitin-like protein, NEDD8, which can be conjugated to cellular proteins, such as Cdc53/culin. [provided by RefSeq, Jul 2008]
- Alternative Names
- Ubiquitin Conjugating Enzyme E2 M; Ubiquitin-Conjugating Enzyme E2M (UBC12 Homolog, Yeast); NEDD8 Carrier Protein; NEDD8 Protein Ligase; UBC12; Ubiquitin-Conjugating Enzyme E2M (Homologous To Yeast UBC12); Ubiquitin-Conjugating Enzyme E2 M; Ubiquitin-Conjugating Enzyme E2M; Ubiquitin Conjugating Enzyme E2M;
- Gene ID
- 9040
- UniProt ID
- P61081
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Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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See other products for "UBE2M"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2123CT | Recombinant Mouse anti-Human UBE2M Monoclonal antibody (EML822) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3759 | Rabbit Anti-UBE2M Recombinant Antibody (clone DS3759AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC804 | AbPlus™ Anti-UBE2M Magnetic Beads (VS-0724-YC804) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY528 | Mouse Anti-NHP UBE2M Recombinant Antibody (clone OTI2D9) | WB, ICC | Mouse IgG1 |
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