Mouse Anti-NHP UBE2M Recombinant Antibody (clone OTI2D9)
CAT#: VS-1024-XY528
Harnessing the specificity for UBE2M, this antibody is developed from a full-length human recombinant protein, ensuring robust reactivity with dog, human, mouse, and non-human primate samples. Its applications in Western Blotting (WB) and Immunocytochemistry (ICC) enable researchers to explore the complexities of ubiquitination and its critical roles in cellular functions. Renowned for its exceptional sensitivity and reliability, this antibody is an indispensable tool for advancing knowledge of UBE2M's involvement in protein degradation pathways and their significance in various physiological and pathological contexts.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, nuclear bodies & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/1272_D2_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm & nucleoli.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57800/1147_E12_3_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows distinct cytoplasmic and nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/57800/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong nuclear and moderate cytoplasmic positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4993/ihc_selected.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Leydig cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/137512_A_6_6.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Nuclear Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54551/137512_A_9_3.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HEK93)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130725-UBE2M
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Specifications
- Immunogen
- Full length human recombinant protein of UBE2M
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- UBE2M
- Species Reactivity
- Dog, Human, Mouse, Non-human primate
- Clone
- OTI2D9
- Applications
- WB, ICC
Product Property
- Purification
- Affinity Chromatography
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, ICC: 1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
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COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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| CAT | Product Name | Application | Type |
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| MOB-2123CT | Recombinant Mouse anti-Human UBE2M Monoclonal antibody (EML822) | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3759 | Rabbit Anti-UBE2M Recombinant Antibody (clone DS3759AB) | WB, IHC, ICC, IP, FC | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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