Rabbit Anti-UBE4B Recombinant Antibody (clone R04-6C6) (CAT#: VS3-FY2100)
Clone R04-6C6 is a rabbit recombinant monoclonal antibody that has been meticulously crafted to specifically bind to human UBE4B. It cross-reacts with rat. The ubiquitin conjugation factor E4 B antibody has been teted positive by western blot analysis of UBE4B in PC-12, C6 lysates and western blot analysis of UBE4B in Hela, A549, HL-60, U2OS, U897MG lysates.
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WB
Figure 1 Anti-UBE4B Antibody (VS3-FY2100) in WB.
The UBE4B expression in PC-12, C6 lysates were detected using the anti-Ubiquitin Conjugation Factor E4 B by western blot assay.
WB
Figure 2 Anti-UBE4B Antibody (VS3-FY2100) in WB.
The UBE4B expression in Hela, A549, HL-60, U2OS, U897MG lysates were detected using the anti-UBE4B by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human UBE4B
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat UBE4B
- Species Reactivity
- Human, Rat
- Clone
- R04-6C6
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 146 kDa, while the observed molecular weight stands at 146 kDa.
- Related Disease
- Chromosome 1P36 Deletion Syndrome and Neuroblastoma 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ubiquitination Factor E4B; Ubiquitination Factor E4B (UFD2 Homolog, Yeast); RING-Type E3 Ubiquitin Transferase E4 B; HDNB1; UFD2; Ubiquitination Factor E4B (Homologous To Yeast UFD2); Homozygously Deleted In Neuroblastoma-1; Homozygously Deleted In Neuroblastoma 1; Ubiquitin-Fusion Degradation Protein 2; Ubiquitin Fusion Degradation Protein 2
- Gene ID
- 10277
- UniProt ID
- O95155
- Sequence Similarities
- Belongs to the ubiquitin conjugation factor E4 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Proteolytically cleaved by caspases during apoptosis.
Cleaved efficiently at Asp-123 by caspase-6 and granzyme B.
Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
(O95155-UBE4B_HUMAN)
Ubiquitination at Lys610, Lys794, Lys801, Lys951, Lys964, Lys989, Lys1106, and Lys1189 (NX_O95155 [NX_O95155-1])
Modification sites at PhosphoSitePlus (O95155)
Glycosylation from GlyGen (O95155) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_011538790.1; XP_011538791.1; XP_011538792.1; XP_016855524.1; XP_047283974.1
- Function
- May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity).
May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2129CT | Recombinant Mouse anti-Human UBE4B Monoclonal antibody (EML1066) | WB | |
| VS13-YC1203 | Rabbit Anti-UBE4B Recombinant Antibody (VS13-YC1203) | WB, ICC, IF | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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