Rabbit Anti-UBE4B Recombinant Antibody (clone R04-6C6)
CAT#: VS3-FY2100
Clone R04-6C6 is a rabbit recombinant monoclonal antibody that has been meticulously crafted to specifically bind to human UBE4B. It cross-reacts with rat. The ubiquitin conjugation factor E4 B antibody has been teted positive by western blot analysis of UBE4B in PC-12, C6 lysates and western blot analysis of UBE4B in Hela, A549, HL-60, U2OS, U897MG lysates.
Tested Data
Gene Expression
WB
Figure 1 Anti-UBE4B Antibody (VS3-FY2100) in WB.
The UBE4B expression in PC-12, C6 lysates were detected using the anti-Ubiquitin Conjugation Factor E4 B by western blot assay.
WB
Figure 2 Anti-UBE4B Antibody (VS3-FY2100) in WB.
The UBE4B expression in Hela, A549, HL-60, U2OS, U897MG lysates were detected using the anti-UBE4B by western blot assay.
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Normal Tissue
Figure 1 IHC staining of human pancreas
Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19219/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19219/45197_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19219/45197_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Cells in tubules Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19219/45197_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19219/45197_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19219/45197_A_9_8.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000130939-UBE4B
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Specifications
- Immunogen
- Synthetic peptide of human UBE4B
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat UBE4B
- Species Reactivity
- Human, Rat
- Clone
- R04-6C6
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 146 kDa, while the observed molecular weight stands at 146 kDa.
- Related Disease
- Chromosome 1P36 Deletion Syndrome and Neuroblastoma 1
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- Ubiquitination Factor E4B; Ubiquitination Factor E4B (UFD2 Homolog, Yeast); RING-Type E3 Ubiquitin Transferase E4 B; HDNB1; UFD2; Ubiquitination Factor E4B (Homologous To Yeast UFD2); Homozygously Deleted In Neuroblastoma-1; Homozygously Deleted In Neuroblastoma 1; Ubiquitin-Fusion Degradation Protein 2; Ubiquitin Fusion Degradation Protein 2
- Gene ID
- 10277
- UniProt ID
- O95155
- Sequence Similarities
- Belongs to the ubiquitin conjugation factor E4 family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Proteolytically cleaved by caspases during apoptosis.
Cleaved efficiently at Asp-123 by caspase-6 and granzyme B.
Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7.
(O95155-UBE4B_HUMAN)
Ubiquitination at Lys610, Lys794, Lys801, Lys951, Lys964, Lys989, Lys1106, and Lys1189 (NX_O95155 [NX_O95155-1])
Modification sites at PhosphoSitePlus (O95155)
Glycosylation from GlyGen (O95155) 2 sites, 1 O-linked glycan (2 sites)
- Protein Refseq
- XP_011538790.1; XP_011538791.1; XP_011538792.1; XP_016855524.1; XP_047283974.1
- Function
- May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity).
May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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