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    Mouse Anti-UCHL1 (C-Terminus) Recombinant Antibody (VS3-CJ549) (CAT#: VS3-CJ549)

    This product is a mouse antibody that recognizes human, mouse, and rat UCHL1 (C-Terminus).

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    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
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    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line SH-SY5Y
    Figure 1 IF staining of human cell line SH-SY5Y
    (Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/5993/1902_A11_2_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/665_E11_1_selected.jpg
    Normal Tissue
    Figure 3 Kidney
    Figure 3 Kidney
    (Collecting ducts Staining: Low Intensity: Moderate Quantity: <25% Distal tubules Staining: High Intensity: Strong Quantity: 75%-25%)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_8_5.jpg
    Figure 4 Testis
    Figure 4 Testis
    (Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Spermatogonia cells Staining: Medium Intensity: Moderate)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_6_6.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_B_8_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25% Peripheral nerve/ganglion Staining: High Intensity: Strong)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_9_3.jpg
    Figure 7 Pancreas
    Figure 7 Pancreas
    (Pancreatic endocrine cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/2580/6513_A_1_3.jpg
    RNA Expression
    Figure 8 RNA cell line category: Cell line enhanced (EFO-21, SCLC-21H, U-251 MG, U-266/84, U-87 MG)
    Figure 8 RNA cell line category: Cell line enhanced (EFO-21, SCLC-21H, U-251 MG, U-266/84, U-87 MG)
    (Cell lines ordered by descending RNA expression order)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000154277-UCHL1

    Specifications

    • Immunogen
    • KLH-conjugated synthetic peptide selected from the 185-214 region of human UCHL1
    • Host Species
    • Mouse
    • Type
    • Mouse IgG1
    • Specificity
    • Human, Mouse, Rat UCHL1 (C-Terminus)
    • Species Reactivity
    • Human, Mouse, Rat
    • Applications
    • WB
    • Conjugate
    • Unconjugated

    Product Property

    • Purification
    • Protein G affinity purified
    • Purity
    • >95% as determined by SDS-PAGE
    • Format
    • Liquid
    • Buffer
    • PBS, pH 7.3.
    • Preservative
    • 0.09% Sodium Azide
    • Storage
    • Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

    Applications

    • Application Notes
    • This antibody has been tested for use in Western Blot (1:1000).

    Target

    • Alternative Names
    • NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
    • Sequence Similarities
    • Belongs to the peptidase C12 family.
    • Cellular Localization
    • Cytoplasm, Endoplasmic reticulum, Membrane
    • Post Translation Modifications
    • O-glycosylated.
    • Function
    • Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Recommended Dilution Buffer

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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