Mouse Anti-UQCRC1 Recombinant Antibody (clone 1A3D12) (CAT#: VS7-0425-WR1100)

• Immunogen
• Recombinant human UQCRC1 (AA: 60-227) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG1

• Specificity
• Human UQCRC1

• Species Reactivity
• Human

• Clone
• 1A3D12

• Molecule Class
• Mitochondrial Ubiquinol-Cytochrome C Reductase Complex Subunit

• Applications
• WB, IHC, ICC, FC

• Conjugate
• Unconjugated

• Related Disease
• Parkinsonism with polyneuropathy (PKNPY)

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry-Paraffin: 1:200 - 1:1000
  Immunocytochemistry: 1:50 - 1:200
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• Ubiquinol-Cytochrome C Reductase Core Protein 1; Ubiquinol-Cytochrome-C Reductase Complex Core Protein 1; Ubiquinol-Cytochrome C Reductase Core Protein I; Complex III Subunit 1; Core Protein I; Cytochrome B-C1 Complex Subunit 1, Mitochondrial; D3S3191; UQCR1; QCR1

• Gene ID
• 7384

• UniProt ID
• P31930

• Long Name
• Ubiquinol-Cytochrome C Reductase Core Protein 1

• Cellular Localization
• Membrane; Mitochondrion; Mitochondrion inner membrane

• Post Translation Modifications
• Acetylation; Phosphoprotein

• Protein Refseq
• NP_003356.2

• Function
• Component of the ubiquinol-cytochrome c oxidoreductase, a multisubunit transmembrane complex that is part of the mitochondrial electron transport chain which drives oxidative phosphorylation. The respiratory chain contains 3 multisubunit complexes succinate dehydrogenase (complex II, CII), ubiquinol-cytochrome c oxidoreductase (cytochrome b-c1 complex, complex III, CIII) and cytochrome c oxidase (complex IV, CIV), that cooperate to transfer electrons derived from NADH and succinate to molecular oxygen, creating an electrochemical gradient over the inner membrane that drives transmembrane transport and the ATP synthase. The cytochrome b-c1 complex catalyzes electron transfer from ubiquinol to cytochrome c, linking this redox reaction to translocation of protons across the mitochondrial inner membrane, with protons being carried across the membrane as hydrogens on the quinol. In the process called Q cycle, 2 protons are consumed from the matrix, 4 protons are released into the intermembrane space and 2 electrons are passed to cytochrome c (By similarity).; The 2 core subunits UQCRC1/QCR1 and UQCRC2/QCR2 are homologous to the 2 mitochondrial-processing peptidase (MPP) subunits beta-MPP and alpha-MPP respectively, and they seem to have preserved their MPP processing properties (By similarity).; May be involved in the in situ processing of UQCRFS1 into the mature Rieske protein and its mitochondrial targeting sequence (MTS)/subunit 9 when incorporated into complex III (Probable). Seems to play an important role in the maintenance of proper mitochondrial function in nigral dopaminergic neurons (PubMed:33141179).

Isotype Control

• MOB-070CQ
• Goat anti-Mouse IgG Fc Antibody, HRP

Secondary Antibody

• 2RD-A1562-YJ
• Rabbit Anti-Mouse IgG1 Secondary antibody (Clone RM106)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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