Mouse Anti-USP2 Recombinant Antibody (VS3-CJ180) (CAT#: VS3-CJ180)

• Immunogen
• Recombinant protein between 1-258 amino acids from human USP2

• Host Species
• Mouse

• Specificity
• Human USP2

• Species Reactivity
• Human

• Applications
• WB

• Conjugate
• Unconjugated

• Purification
• Affinity purified

• Purity
• >95% as determined by SDS-PAGE

• Buffer
• PBS

• Preservative
• 0.09% sodium azide

• Storage
• Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• This antibody has been tested for use in Western Blot (1:2000).

• Alternative Names
• USP9; UBP41

• Gene ID
• 9099

• UniProt ID
• O75604

• Sequence Similarities
• Belongs to the peptidase C19 family. USP2 subfamily.

• Cellular Localization
• Cytoplasm, Membrane, Nucleus

• Protein Refseq
• NP_001230688.1; NP_004196.4; NP_741994.1

• Function
• Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (PubMed:17290220, PubMed:19917254, PubMed:19838211).
  Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities (By similarity).
  Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (PubMed:17290220, PubMed:19838211).
  Has no deubiquitinase activity against p53/TP53 (PubMed:17290220).
  Prevents MDM2-mediated degradation of MDM4 (PubMed:17290220).
  Plays a role in the G1/S cell-cycle progression in normal and cancer cells (PubMed:19917254).
  Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (By similarity).
  Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (By similarity).
  Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (By similarity).
  Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity).
  Isoform 4: Circadian clock output effector that regulates Ca2+ absorption in the small intestine. Probably functions by regulating protein levels of the membrane scaffold protein NHERF4 in a rhythmic manner, and is therefore likely to control Ca2+ membrane permeability mediated by the Ca2+ channel TRPV6 in the intestine.

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS