Rabbit Anti-WARS Recombinant Antibody (clone R06-5I7)
CAT#: VS3-FY2097
This R06-5I7 recombinant monoclonal antibody is specifically created to target human WARS. It cross-reacts with mouse, rat. This tryptophan tRNA Ligase antibody was verified by western blot analysis of tryptophanyl tRNA synthetase/WRS in mouse brain, rat brain lysates and western blot analysis of Tryptophanyl tRNA synthetase/WRS in Hela, A549, HL-60 lysates.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/159_A1_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18944/if_selected.jpg
Normal Tissue
Figure 3 Placenta
High expression in placenta
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_A_3_7_val_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HHSteC, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140105-WARS
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WB
Figure 1 Anti-WARS Antibody (VS3-FY2097) in WB.
The Tryptophanyl tRNA synthetase/WRS expression in mouse brain, rat brain lysates were detected using the anti-Tryptophan tRNA Ligase by western blot assay.
WB
Figure 2 Anti-WARS Antibody (VS3-FY2097) in WB.
The Tryptophanyl tRNA synthetase/WRS expression in Hela, A549, HL-60 lysates were detected using the anti-Tryptophanyl tRNA synthetase/WRS by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human tryptophanyl-tRNA synthetase/WRS
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat WARS
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-5I7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 53 kDa.
- Related Disease
- Neuronopathy, Distal Hereditary Motor, Autosomal Dominant 9 and Neurodevelopmental Disorder With Microcephaly And Speech Delay, With Or Without Brain Abnormalities
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Tryptophanyl-TRNA Synthetase; Tryptophan TRNA Ligase 1, Cytoplasmic; Interferon-Induced Protein 53; EC 6.1.1.2; TrpRS; IFI53; IFP53
- Gene ID
- 7453
- UniProt ID
- P23381
- Sequence Similarities
- Belongs to the class-I aminoacyl-tRNA synthetase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. (P23381-SYWC_HUMAN)
Ubiquitination at Lys102 and Lys154 (NX_P23381 [NX_P23381-1])
Modification sites at PhosphoSitePlus (P23381)
Glycosylation from GlyGen (P23381) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011535438.1; XP_016877116.1; XP_024305475.1; XP_047287713.1; XP_047287714.1
- Function
- Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "WARS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2063z | Mouse Anti-WARS Recombinant Antibody (clone 31B5) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
| ZG-442C | Human Anti-WARS Recombinant Antibody (ZG-442C) | ELISA, IP | Human IgG1, κ |
| VS13-YC1225 | Rabbit Anti-WARS Recombinant Antibody (VS13-YC1225) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3862 | Rabbit Anti-WARS Recombinant Antibody (clone DS3862AB) | ICC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC552 | AbPlus™ Anti-WARS Magnetic Beads (VS-0724-YC552) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2483 | Anti-WARS Immunohistochemistry Kit | IHC | |
| VS-0525-XY7889 | Anti-Human WARS Immunohistochemistry Kit | IHC |
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