Rabbit Anti-WARS Recombinant Antibody (clone R06-5I7) (CAT#: VS3-FY2097)
This R06-5I7 recombinant monoclonal antibody is specifically created to target human WARS. It cross-reacts with mouse, rat. This tryptophan tRNA Ligase antibody was verified by western blot analysis of tryptophanyl tRNA synthetase/WRS in mouse brain, rat brain lysates and western blot analysis of Tryptophanyl tRNA synthetase/WRS in Hela, A549, HL-60 lysates.
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WB
Figure 1 Anti-WARS Antibody (VS3-FY2097) in WB.
The Tryptophanyl tRNA synthetase/WRS expression in mouse brain, rat brain lysates were detected using the anti-Tryptophan tRNA Ligase by western blot assay.
WB
Figure 2 Anti-WARS Antibody (VS3-FY2097) in WB.
The Tryptophanyl tRNA synthetase/WRS expression in Hela, A549, HL-60 lysates were detected using the anti-Tryptophanyl tRNA synthetase/WRS by western blot assay.
Specifications
- Immunogen
- Synthetic peptide of human tryptophanyl-tRNA synthetase/WRS
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat WARS
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R06-5I7
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- MW
- The calculated weight of the target protein is approximately 53 kDa, while the observed molecular weight stands at 53 kDa.
- Related Disease
- Neuronopathy, Distal Hereditary Motor, Autosomal Dominant 9 and Neurodevelopmental Disorder With Microcephaly And Speech Delay, With Or Without Brain Abnormalities
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/50-1/100
Target
- Alternative Names
- Tryptophanyl-TRNA Synthetase; Tryptophan TRNA Ligase 1, Cytoplasmic; Interferon-Induced Protein 53; EC 6.1.1.2; TrpRS; IFI53; IFP53
- Gene ID
- 7453
- UniProt ID
- P23381
- Sequence Similarities
- Belongs to the class-I aminoacyl-tRNA synthetase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Proteolytic cleavage generates 2 forms; T1-TrpRS and T2-TrpRS. (P23381-SYWC_HUMAN)
Ubiquitination at Lys102 and Lys154 (NX_P23381 [NX_P23381-1])
Modification sites at PhosphoSitePlus (P23381)
Glycosylation from GlyGen (P23381) 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- XP_011535438.1; XP_016877116.1; XP_024305475.1; XP_047287713.1; XP_047287714.1
- Function
- Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2063z | Mouse Anti-WARS Recombinant Antibody (clone 31B5) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
| ZG-442C | Human Anti-WARS Recombinant Antibody (ZG-442C) | ELISA, IP | Human IgG1, κ |
| VS13-YC1225 | Rabbit Anti-WARS Recombinant Antibody (VS13-YC1225) | WB, IHC-Fr, IHC-P, ICC, IF, IP | Rabbit IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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