Rabbit Anti-WARS Recombinant Antibody (VS13-YC1225)
CAT#: VS13-YC1225
The Rabbit Anti-WARS Recombinant Antibody is specific for human WARS. WARS encodes tryptophanyl-tRNA synthetase, catalyzing tRNA aminoacylation with tryptophan and is induced by interferon. The anti-WARS antibody can be used for Western blot (1/500-1/1000), Immunohistochemistry (1/50-1/100), Immunofluorescence (1/50-1/200), Immunoprecipitation (1/20).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/159_A1_1_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18944/if_selected.jpg
Normal Tissue
Figure 3 Placenta
High expression in placenta
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_A_3_7_val_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5573/15468_A_6_6.jpg
RNA Expression
Figure 8 RNA cell line category: Cell line enhanced (HHSteC, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000140105-WARS
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Specifications
- Immunogen
- Recombinant human Tryptophanyl tRNA synthetase.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human WARS
- Species Reactivity
- Human
- Applications
- WB, IHC-Fr, IHC-P, ICC, IF, IP
- Conjugate
- NONE
- Related Disease
- Neuronopathy, Distal Hereditary Motor, Autosomal Dominant and Neurodevelopmental Disorder With Microcephaly And Speech Delay, With Or Without Brain Abnormalities
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity Chromatography purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4) containing 150 mM NaCl, 40% Glycerol and 0.05% BSA.
- Preservative
- 0.01% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/1000
Immunohistochemistry (IHC): 1/50-1/100
Immunofluorescence (IF): 1/50-1/200
Immunoprecipitation (IP): 1/20
Target
- Alternative Names
- Tryptophanyl-TRNA Synthetase; Tryptophan TRNA Ligase 1, Cytoplasmic; Interferon-Induced Protein 53; EC 6.1.1.2; TrpRS; IFI53; IFP53;
- Gene ID
- 7453
- UniProt ID
- P23381
- Long Name
- Tryptophanyl-TRNA Synthetase
- Sequence Similarities
- Belongs to the class-I aminoacyl-tRNA synthetase family.
- Cellular Localization
- Cytoplasm
- Protein Refseq
- XP_011535438.1; XP_016877116.1; XP_024305475.1; XP_047287713.1; XP_047287714.1
- Function
- Catalyzes the attachment of tryptophan to tRNA(Trp) in a two-step reaction: tryptophan is first activated by ATP to form Trp-AMP and then transferred to the acceptor end of the tRNA(Trp).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "WARS"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2063z | Mouse Anti-WARS Recombinant Antibody (clone 31B5) | WB, ELISA, IF, IHC, IP | Mouse IgG2b, κ |
| ZG-442C | Human Anti-WARS Recombinant Antibody (ZG-442C) | ELISA, IP | Human IgG1, κ |
| VS3-FY2097 | Rabbit Anti-WARS Recombinant Antibody (clone R06-5I7) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3862 | Rabbit Anti-WARS Recombinant Antibody (clone DS3862AB) | ICC, IF, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC552 | AbPlus™ Anti-WARS Magnetic Beads (VS-0724-YC552) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2483 | Anti-WARS Immunohistochemistry Kit | IHC | |
| VS-0525-XY7889 | Anti-Human WARS Immunohistochemistry Kit | IHC |
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