Rabbit Anti-WTAP Recombinant Antibody (VS3-FY2919)

CAT#: VS3-FY2919

The rabbit anti-WTAP recombinant monoclonal antibody has high affinity for human WTAP protein. This monoclonal antibody proves valuable in disease research related to Wilms Tumor 5 and Wilms Tumor 1. The optimal dilution for this antibody is Immunoprecipitation (1/20), Western Blot (1/500-1/1000), Immunohistochemistry (1/50-1/100).

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human WTAP
  • Species Reactivity
  • Human
  • Applications
  • Western Blot, Immunohistochemistry, Immunoprecipitation
  • Conjugate
  • Unconjugated, FITC
  • Related Disease
  • Wilms Tumor 5 and Wilms Tumor 1

Product Property

  • Clonality
  • Monoclonal Antibody
  • Format
  • Liquid
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

Applications

  • Application Notes
  • Immunoprecipitation: 1/20
    Western Blot: 1/500-1/1000
    Immunohistochemistry: 1/50-1/100

Target

  • Alternative Names
  • WT1 Associated Protein; Wilms Tumor 1-Associating Protein; Wilms Tumor 1 Associated Protein; Female-Lethal(2)D Homolog; HFL(2)D; Putative Pre-MRNA Splicing Regulator Female-Lethal(2D); Wilms Tumour 1-Associating Protein
  • Sequence Similarities
  • Belongs to the fl(2)d family.
  • Cellular Localization
  • Nucleus speckle
    Nucleus, nucleoplasm
    Cytoplasm
  • Post Translation Modifications
  • Ubiquitination at Lys83 and Lys98 (NX_Q15007 [NX_Q15007-1])
    Modification sites at PhosphoSitePlus (Q15007)
    Glycosylation from GlyGen (Q15007) 1 site, 1 O-linked glycan (1 site)
  • Function
  • Acts as a mRNA splicing regulator (PubMed:12444081).
    Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability (PubMed:17088532).
    Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.
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Downloadable Resources

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Secondary Antibody

Isotype Control

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