Rabbit Anti-XRCC1 Recombinant Antibody (clone R02-0B0)
CAT#: VS3-FY2103
This R02-0B0 recombinant monoclonal antibody is specifically engineered to detect human XRCC1. It cross-reacts with rat. Applications Tested: The XRCC1 antibody has been tested by western blot analysis of XRCC1 in PC-12 lysates and western blot analysis of XRCC1 in A549, MCF-7, 293T lysates.
Tested Data
WB
Figure 1 Anti-XRCC1 Antibody (VS3-FY2103) in WB.
The XRCC1 expression in PC-12 lysates were detected using the anti-XRCC1 by western blot assay.
WB
Figure 2 Anti-XRCC1 Antibody (VS3-FY2103) in WB.
The XRCC1 expression in A549, MCF-7, 293T lysates were detected using the anti-XRCC1 by western blot assay.
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Specifications
- Immunogen
- Synthetic peptide of human XRCC1
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Rat XRCC1
- Species Reactivity
- Human, Rat
- Clone
- R02-0B0
- Applications
- Western Blot, Immunofluorescence
- MW
- The calculated weight of the target protein is approximately 69 kDa, while the observed molecular weight stands at 82 kDa.
- Related Disease
- Cancer
Product Property
- Purification
- Affinity Purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunofluorescence: 1/50-1/200
Target
- Alternative Names
- X-Ray Repair Cross Complementing 1; XRCC1
- Gene ID
- 7515
- UniProt ID
- P18887
- Cellular Localization
- Chromosome, Nucleus
- Post Translation Modifications
- Phosphorylation of Ser-371 causes dimer dissociation.
Phosphorylation by CK2 promotes interaction with APTX and APLF.
(P18887-XRCC1_HUMAN)
Sumoylated. (P18887-XRCC1_HUMAN)
Glycosylation at Thr296, Thr440, Ser446, Ser447, and Thr453 (NX_P18887 [NX_P18887-1])
Modification sites at PhosphoSitePlus (P18887)
Glycosylation from GlyGen (P18887) 6 sites, 2 O-linked glycans (6 sites)
- Protein Refseq
- NP_006288.2
- Function
- Negatively regulates ADP-ribosyltransferase activity of PARP1 during base-excision repair in order to prevent excessive PARP1 activity.
Recognizes and binds poly-ADP-ribose chains: specifically binds auto-poly-ADP-ribosylated PARP1, limiting its activity.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "XRCC1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3628z | Mouse Anti-XRCC1 Recombinant Antibody (clone 41H8) | WB, IP, IF, IHC, ELISA | Mouse IgG2b |
| MRO-1606-CN | Rabbit Anti-XRCC1 Recombinant Antibody (clone JA11-47) | WB, IHC | Rabbit IgG |
| ZG-0513F | Mouse Anti-XRCC1 Recombinant Antibody (ZG-0513F) | WB | Mouse IgG |
| VS3-QX1251 | Mouse Anti-XRCC1 Recombinant Antibody (clone 3F6-G7-F7) | WB | Mouse IgG1 |
| VS7-0425-WR1136 | Mouse Anti-XRCC1 Recombinant Antibody (clone 7B4C12) | WB, IHC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3893 | Rabbit Anti-XRCC1 Recombinant Antibody (clone DS3893AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC167 | AbPlus™ Anti-XRCC1 Magnetic Beads (VS-0724-YC167) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY7965 | Anti-XRCC1 Immunohistochemistry Kit | IHC |
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