Mouse Anti-YWHAB Recombinant Antibody (clone 5B5G10) (CAT#: VS7-0425-WR1143)

• Immunogen
• Recombinant human YWHAB (AA: 1-246) fragment expressed in E. Coli.

• Host Species
• Mouse

• Type
• Mouse IgG2b

• Specificity
• Human YWHAB

• Species Reactivity
• Human, Mouse, Rat

• Clone
• 5B5G10

• Molecule Class
• Phosphoserine/Phosphothreonine-Binding Protein

• Applications
• WB, IHC, ICC, FC

• Conjugate
• Unconjugated

• Clonality
• Monoclonal

• Purity
• >95% as determined by SDS-PAGE and SEC-HPLC analysis

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• PBS

• Preservative
• 0.05% sodium azide

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Ice packs

• Application Notes
• Western Blot: 1:500 - 1:2000
  Immunohistochemistry-Paraffin: 1:200 - 1:1000
  Immunocytochemistry: 1:200 - 1:1000
  Flow Cytometry: 1:200 - 1:400
  Enzyme-linked Immunosorbent Assay: 1:10000

• Alternative Names
• Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Alpha Polypeptide; Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein, Beta Polypeptide; 14-3-3 Alpha; Protein 1054; KCIP-1; Protein Kinase C Inhibitor Protein-1; Protein Kinase C Inhibitor Protein 1; Brain Protein 14-3-3, Beta Isoform

• Gene ID
• 7529

• UniProt ID
• P31946

• Long Name
• Tyrosine 3-Monooxygenase/Tryptophan 5-Monooxygenase Activation Protein Beta

• Cellular Localization
• Cytoplasm; Membrane; Vacuole

• Post Translation Modifications
• Acetylation; Isopeptide bond; Nitration; Phosphoprotein; Ubl conjugation

• Protein Refseq
• NP_003395.1

• Function
• Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.

Isotype Control

• MOB-203CQ
• Mouse IgG2b, kappa Isotype Control Antibody

Secondary Antibody

• 2RD-A1466-YJ
• Goat Anti-Mouse IgG2b Secondary antibody

• 2RD-A1560-YJ
• Rabbit Anti-Mouse IgG2b Secondary antibody (Clone RM108)

Reconstitution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

Recommended Dilution Buffer

• MRO-0253YJ
• Reconstitution Buffer PBS

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