CytoStream™ Rat Anti-IgG1 Isotype Control Recombinant Antibody (RMC-I1030)
CAT#: VS-0126-XU269
CytoStream™ Rat Anti-IgG1 Isotype Control Recombinant Antibody (RMC-I1030) is a high-purity, non-reactive monoclonal reagent designed to serve as a negative control for rat-derived primary antibodies. This recombinant antibody matches the Rat IgG1 subclass but lacks specificity for known mammalian antigens. Its recombinant manufacture ensures the absence of hybridoma-derived impurities and guarantees absolute lot-to-lot consistency. It is specifically validated for flow cytometry (FC) to help researchers confidently define gating thresholds in multi-parameter panels.
Gene Expression
Normal Tissue
Figure 1 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_3.jpg
Normal Tissue
Figure 2 Liver
Hepatocytes
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_9_4.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_8_5.jpg
Normal Tissue
Figure 4 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1245/3618_A_5_6.jpg
RNA Expression
Figure 5 RNA cell line category: Group enriched (Karpas-707, U-698)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000211896-IGHG1
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| Sub Cat | Product Name | Size | Formulation | Conjugate Group | Characteristics | Stability | Size | Conjugation | Endotoxin | Purity | Fc Engineering | |
| VS-0126-XU269-1 | CytoStream™ Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030), PE-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE | Phycobiliprotein fluorochrome (Ex/Em peak: 565/575 nm). One of the brightest fluorophores available, ideal for low-abundance antigen detection. Broad emission requires careful compensation. | Moderate stability. Sensitive to light, heat, and harsh fixatives. Store at 4°C in the dark. Avoid freeze-thaw cycles to maintain performance. | ||||||
| VS-0126-XU269-2 | CytoStream™ Purified Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030) | 100 ug | None | The native antibody without any fluorophore. Used as a primary antibody in indirect staining or for functional assays (blocking, neutralization). Requires a compatible fluorescent secondary antibody for detection. | Typically exhibits the highest intrinsic stability among all formats. Storage conditions are defined by the antibody's own formulation buffer. | |||||||
| VS-0126-XU269-3 | CytoStream™ Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030), PE-Cy5-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy5 | Tandem dye where PE transfers energy to Cy5 (Em ~667 nm). Critical: Use a Cy5 detector, not a PE filter. Prone to degradation of the tandem bond, causing uncoupled PE signal. | Low stability. Highly sensitive to fixation, light, and prolonged storage. Protect from light and analyze fixed samples promptly. | ||||||
| VS-0126-XU269-4 | CytoStream™ Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030), FITC-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | FITC | Small organic fluorophore (Ex/Em: 488/519 nm). High initial brightness but susceptible to photobleaching. Requires careful compensation with PE channel. | Sensitive to light and pH. Prone to rapid photobleaching during acquisition. Store at 4°C in the dark. Prepare working solutions fresh. | ||||||
| VS-0126-XU269-5 | CytoStream™ Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030), AF Dye (488nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (488nm) | Synthetic green fluorogenic dye (Ex/Em ~490/520 nm). A photostable alternative to FITC with brighter signal and narrower emission spectrum, reducing spillover into PE channel. | Excellent photostability. Maintains signal integrity during prolonged acquisition. Tolerates fixation well and exhibits minimal pH sensitivity. | ||||||
| VS-0126-XU269-6 | CytoStream™ Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030), AF Dye (647nm)-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | AF Dye (647nm) | Synthetic far-red fluorogenic dye (Ex/Em ~650/665 nm). Spectrally similar to APC but with enhanced brightness and photostability. Ideal for replacing APC in demanding protocols. | Superior stability. Highly resistant to photobleaching and pH fluctuations. Compatible with harsh fixation and permeabilization protocols. | ||||||
| VS-0126-XU269-7 | CytoStream™ Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030), PE-Cy7-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | PE-Cy7 | Tandem dye (PE excited, Cy7 emits at ~785 nm). Critical for deep-red infrared detection. Essential: Use a Cy7 (780/60 nm) detector, not a PE filter. Susceptible to tandem bond degradation. | Low stability. Highly sensitive to fixation, light exposure, and storage time. Protect from light and analyze immediately post-fixation. | ||||||
| VS-0126-XU269-8 | CytoStream™ Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030), Percp-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Percp | Protein-based fluorochrome (Ex/Em: 488/678 nm). Compatible with blue laser excitation, providing signal in red channel. Lower brightness compared to PE or APC but useful for spectral separation. | Moderate stability. PerCP is inherently photolabile and prone to degradation upon light exposure. Store strictly protected from light. | ||||||
| VS-0126-XU269-9 | CytoStream™ Rat Anti-IgG1 isotype Control Recombinant Antibody (RMC-I1030), Pacific Blue-conjugated | 25 Tests | Formulated in phosphate-buffered saline (PBS) containing 0.2% bovine serum albumin (BSA) and 0.1% Proclin 300, with pH adjusted to 7.2-7.4. | Pacific Blue | Synthetic blue fluorescent dye (Ex/Em: ~410/455 nm). Excellent for violet laser (405 nm) excitation. Low spectral overlap with green/red channel fluorophores, simplifying panel design. | High photostability. Tolerates common fixation protocols well. Store at 4°C in the dark for long-term stability. |
More Infomation
Specifications
- Host Species
- Rat
- Type
- Monoclonal Antibody
- Specificity
- IgG1 isotype Control
- Applications
- FC
Product Property
- Clonality
- Monoclonal
- Storage
- Store at 4˚C.
- Shipping
- Ice Packs
Applications
- Application Notes
- Test: 5 μl/Test
Notice: This reagent is supplied in a pre-optimized, ready-to-use formulation for direct application at the specified volume per test in flow cytometry. The standard protocol is suitable for staining up to 1 million cells per assay. Optimal performance may require protocol adjustments based on specific experimental conditions.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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