Human Anti-CD16 (clone B88-9) scFv-Fc Chimera

CAT#: VS-0325-FY61

Anti-CD16 scFv-Fc antibodies are engineered proteins designed to specifically bind to the CD16 antigen, which is a receptor found on the surface of various immune cells, including natural killer (NK) cells, macrophages, and neutrophils. The term "scFv-Fc" refers to a single-chain variable fragment (scFv) fused to the Fc region of an immunoglobulin. This structure combines the specificity of an antibody's variable region with the effector functions associated with the Fc region, leading to enhanced therapeutic and diagnostic applications.

Specifications

  • Host Species
  • Human
  • Type
  • Human IgG1, scFv-Fc
  • Specificity
  • Human CD16
  • Species Reactivity
  • Human
  • Clone
  • B88-9
  • Applications
  • Immunoprecipitation, Flow Cytometry, Immunofluorescence
  • Related Disease
  • Cancer

Product Property

  • Purity
  • >95% as determined by SDS-PAGE
  • Concentration
  • Please refer to the vial label for the specific concentration.
  • Storage
  • Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
  • Shipping
  • Ice packs

Target

  • Alternative Names
  • CD16; FCGR3
  • Long Name
  • CD16
  • Cellular Localization
  • Cell membrane; Secreted
  • Post Translation Modifications
  • Glycosylated.
    Contains high mannose- and complex-type oligosaccharides.
    Glycosylation at Asn-180 is mandatory for high affinity binding to the Fc and for discrimination between fucosylated and afucosylated IgG glycoforms.
    Undergoes rapid ectodomain shedding upon NK cell stimulation.
    The soluble form is produced by a proteolytic cleavage mediated by ADAM17.
    Repeated stimulation causes receptor shedding, a mechanism that allows for increased NK cell motility and detachment from opsonized target cells while avoiding activation-induced NK cell apoptosis.
    Phosphorylated at RSSTR motif by PKC.
    The relevant physiological PKCs might be PRKCI, PRKCG, PRKCE, PRKCH and PRKCQ.
    Glycosylation at Asn56, Asn63, Asn92, Asn180, and Asn187
    Modification sites at PhosphoSitePlus
    Glycosylation from GlyConnect FCG3A_HUMAN (3000)
    Glycosylation from GlyGen 7 sites, 99 N-linked glycans (6 sites)
  • Function
  • CD16 (Fc gamma receptor III) is an Fc receptor expressed on natural killer cells and neutrophils. It mediates antibody-dependent cellular cytotoxicity (ADCC) and plays a crucial role in the immune response.
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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

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COA

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Protocol & Troubleshooting

We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.

Isotype Control

Secondary Antibody

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