Human Anti-TK1 scFv-Fc Chimera (VS-0325-FY142)
CAT#: VS-0325-FY142
This product is an engineered human scFv-Fc antibody designed to target thymidine kinase 1 (TK1), a cell surface biomarker that is upregulated in various cancers. The antibody, named "Wheelz," was isolated from a yeast display library using magnetic sorting and flow cytometry with TK1 as the target. Wheelz exhibits high specificity for TK1, recognizing its monomeric, dimeric, and tetrameric forms in normal serum, cancer serum, and cancer cell extracts.
Gene Expression
Normal Tissue
Figure 1 Tonsil
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4683/ihc_selected.jpg
Normal Tissue
Figure 2 Placenta
Trophoblastic cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4683/18578_A_1_7.jpg
Normal Tissue
Figure 3 Lymph node
Germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear Non-germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4683/18578_A_8_8.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000167900-TK1
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Specifications
- Immunogen
- Purified TK1 protein.
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human TK1
- Species Reactivity
- Human
- Applications
- Flow Cytometry
- Related Disease
- Cancer
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Target
- Alternative Names
- Thymidine Kinase 1; EC 2.7.1.21; Thymidine Kinase 1 Soluble Isoform; Thymidine Kinase 1, Soluble; Thymidine Kinase, Cytosolic; TK2
- Gene ID
- 7083
- UniProt ID
- P04183
- Long Name
- Thymidine Kinase 1
- Sequence Similarities
- Belongs to the thymidine kinase family.
- Cellular Localization
- Cytoplasm
- Post Translation Modifications
- Phosphorylated on Ser-13 in mitosis.
Phosphorylation of Ser-13 by CDK1 during mitosis reduces homotetramerization and catalytic efficiency when DNA replication is complete and intracellular TK1 is still present at a high level.
Polyubiquitinated.
Postmitosis, ubiquitination leads to proteasomal degradation.
The KEN box sequence located at the C-terminal region targets for degradation by the anaphase promoting complex (APC/C) activated and rate-limited by FZR1.
Ubiquitination at Lys220
Modification sites at PhosphoSitePlus
Glycosylation from GlyGen 1 site, 1 O-linked glycan (1 site)
- Protein Refseq
- NP_001333592.1; NP_001350777.1; NP_003249.3
- Function
- Human TK1 (Thymidine Kinase 1) is an enzyme involved in DNA synthesis, and its activity is elevated in proliferating cells, including cancer cells. It serves as a biomarker for cell proliferation and is used in cancer diagnostics.
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Related Signaling Pathways
Canonical Wnt Signaling Pathway
Non-Canonical Wnt Signaling Pathway
Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "TK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1589z | Mouse Anti-TK1 Recombinant Antibody (clone 32A3) | WB, ELISA | Mouse IgG1 |
| MOB-2030CT | Recombinant Mouse anti-Human TK1 Monoclonal antibody (EML1897) | IHC-P, WB | |
| MRO-1492-CN | Rabbit Anti-TK1 Recombinant Antibody (clone JF0970) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY1456 | Recombinant Rabbit Anti-TK1 Antibody (clone R05-9G6) | WB, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0419CL | Mouse Anti-TK1 Recombinant Antibody (TAB-0419CL) | ELISA | Mouse IgG |
| TAB-0420CL | Mouse Anti-TK1 Recombinant Antibody (TAB-0420CL) | ELISA, DB | Mouse IgG |
| TAB-0421CL | Mouse Anti-TK1 Recombinant Antibody (TAB-0421CL) | ELISA, DB | Mouse IgG |
| TAB-0419CL-S(P) | Mouse Anti-TK1 Recombinant Antibody; scFv Fragment (TAB-0419CL-S(P)) | ELISA | Mouse scFv |
| TAB-0420CL-S(P) | Mouse Anti-TK1 Recombinant Antibody; scFv Fragment (TAB-0420CL-S(P)) | ELISA, DB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3579 | Rabbit Anti-TK1 Recombinant Antibody (clone DS3579AB) | FC, ICC, IF, IHC-P, IP, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2296 | Anti-TK1 Immunohistochemistry Kit | IHC |
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