Human Anti-TNFSF10 (clone DR4-4) scFv-Fc Chimera
CAT#: VS-0425-FY49
The anti-TNFSF10 scFv-Fc fusion protein is designed to target Tumor Necrosis Factor Superfamily Member 10 (TNFSF10), also known as TRAIL. This protein combines a single-chain variable fragment (scFv) for high-specificity binding to TNFSF10, with an Fc domain to enhance its stability and extend its circulating half-life. By targeting TNFSF10, this protein aims to induce apoptosis, or programmed cell death, primarily in tumor cells. The Fc portion of this molecule, works to prolong the time that the protien remaines in the blood stream. This design intends for a therapeutic application, in the fight against certain cancer types. Ongoing research focuses on optimizing its efficacy and safety profile for clinical use.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IHC staining of human kidney
Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in cells in tubules.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54938/ihc_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54938/134466_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54938/134466_A_9_3.jpg
Normal Tissue
Figure 4 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Cytoplasmic/membranous
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54938/134466_A_7_5.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Low
Intensity: Weak
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54938/134466_A_4_6.jpg
Normal Tissue
Figure 6 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54938/134466_A_7_8.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BEWO, CAPAN-2, HaCaT, HMC-1, RPTEC TERT1)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000121858-TNFSF10/subcellular
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Specifications
- Host Species
- Human
- Type
- Human IgG1, scFv-Fc
- Specificity
- Human TNFSF10
- Species Reactivity
- Human
- Clone
- DR4-4
- Applications
- Western Blot, ELISA, Flow Cytometry, Cytotoxicity
- Related Disease
- Tumors
Product Property
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1 μg/mL
Cytotoxicity: 100 μg/mL
The optimal dilution concentration is determined by the experiment.
Target
- Alternative Names
- TNF Superfamily Member 10; Tumor Necrosis Factor (Ligand) Superfamily, Member 10; Apo-2 Ligand; Apo-2L; APO2L; TRAIL; Tumor Necrosis Factor Apoptosis-Inducing Ligand Splice Variant Delta; Chemokine Tumor Necrosis Factor Ligand Superfamily Member 10; Tumor Necrosis Factor Ligand Superfamily Member 10; Tumor Necrosis Factor (Ligand) Family, Member 10
- Gene ID
- 8743
- UniProt ID
- P50591
- Long Name
- TNF Superfamily Member 10
- Sequence Similarities
- Belongs to the tumor necrosis factor family.
- Cellular Localization
- Cell membrane, Membrane, Secreted
- Post Translation Modifications
- Tyrosine phosphorylated by PKDCC/VLK.
- Protein Refseq
- NP_001177871.1; NP_003801.1
- Function
- Cytokine that binds to TNFRSF10A/TRAILR1, TNFRSF10B/TRAILR2, TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and possibly also to TNFRSF11B/OPG.
Induces apoptosis. Its activity may be modulated by binding to the decoy receptors TNFRSF10C/TRAILR3, TNFRSF10D/TRAILR4 and TNFRSF11B/OPG that cannot induce apoptosis.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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