Mouse Anti-BRCA1 Recombinant Antibody (clone 6C6D2)
CAT#: VS3-XY137
Clone 6C6D2 is a mouse antibody, which reacts with human BRCA1 protein. This antibody is suitable for BRCA1 detection by ELISA, WB, IHC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm & nuclear bodies.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4840/1914_E5_15_cr5c8770e98e94d_selected.jpg
Normal Tissue
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows strong nuclear positivity in a subset of cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/34966/89241_A_4_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1946/5290_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1946/5290_A_9_3.jpg
Normal Tissue
Figure 5 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1946/5290_A_6_6.jpg
Normal Tissue
Figure 6 Skin
Epidermal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1946/5290_B_5_8.jpg
Normal Tissue
Figure 7 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
Non-germinal center cells
Staining: Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1946/5290_A_8_8.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000012048-BRCA1
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Specifications
- Immunogen
- Recombinant fragment of human BRCA1 (a.a. 229-335) expressed in E. coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human BRCA1
- Species Reactivity
- Human
- Clone
- 6C6D2
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry
Product Property
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Immunohistochemistry(1/200-1/1000).
Target
- Alternative Names
- BRCA1, DNA Repair Associated; Protein Phosphatase 1, Regulatory Subunit 53; BRCA1/BRCA2-Containing Complex, Subunit 1; Fanconi Anemia, Complementation Group S; Breast Cancer 1, Early Onset; RING Finger Protein 53; RNF53; Breast And Ovarian Cancer Susceptibility Protein 1; Breast Cancer Type 1 Susceptibility Protein; RING-Type E3 Ubiquitin Transferase BRCA1; Early Onset Breast Cancer 1; Breast Cancer 1
- Gene ID
- 672
- UniProt ID
- P38398
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "BRCA1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-123 | Recombinant Anti-human BRCA1 Antibody | ELISA, WB, Neut, FuncS | IgG |
| MHH-123 | Recombinant Human Anti-human BRCA1 Antibody | FC, ELISA, FuncS | IgG |
| MOB-1473MZ | Mouse Anti-BRCA1 Recombinant Antibody (clone 7C5) | IP, WB | Mouse IgG1 |
| VS3-WK1325 | Mouse Anti-BRCA1 Recombinant Antibody (VS3-WK1325) | ELISA, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-123-F(E) | Recombinant Anti-human BRCA1 Antibody Fab Fragment | FC, Neut, Funcs | Fab |
| MHH-123-F(E) | Recombinant Human Anti-human BRCA1 Antibody Fab Fragment | WB, Dot, FuncS | Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-123-S(P) | Recombinant Anti-human BRCA1 Antibody scFv Fragment | ELISA, IP, FuncS | scFv |
| MHH-123-S(P) | Recombinant Human Anti-human BRCA1 Antibody scFv Fragment | WB, IHC, FuncS | scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0071MZ | Chicken Anti-BRCA1 Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY824 | Anti-BRCA1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY825 | Anti-Mouse BRCA1 Immunohistochemistry Kit | IHC |
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