Mouse Anti-CEBPA Recombinant Antibody (clone 5B7) (CAT#: VS3-XY491)
Clone 5B7 is a mouse antibody, which reacts with human CEBPA protein. This antibody is suitable for CEBPA detection by ELISA, WB, FC assays.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line CACO-2
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52734/1549_E6_1_selected.jpg
(Immunofluorescent staining of human cell line CACO-2 shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52734/1549_E6_1_selected.jpg
Figure 2 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67937/1528_A1_1_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67937/1528_A1_1_selected.jpg
Figure 3 IHC staining of human stomach
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/ihc_selected.jpg
(Immunohistochemical staining of human stomach shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/ihc_selected.jpg
Figure 4 Pancreas
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_1_3.jpg
(Exocrine glandular cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_1_3.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/65037/150497_A_9_5.jpg
Figure 6 RNA cell line category: Cell line enhanced (BEWO, Hep G2, NB-4, THP-1, U-937)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000245848-CEBPA
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000245848-CEBPA
Specifications
- Immunogen
- Synthesized peptide of human CEBPA (a.a. C-RKSRDKAKRNVETKV).
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human CEBPA
- Species Reactivity
- Human
- Clone
- 5B7
- Applications
- Enzyme-linked Immunosorbent Assay, Western Blot, Flow Cytometry
Product Property
- Format
- Liquid
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody is recommended for Western Blot (1/500-1/2000), Enzyme-linked Immunosorbent Assay (1/10000) and Flow Cytometry (1/200-1/400).
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kits & Antibodies
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3995z | Mouse Anti-CEBPA Recombinant Antibody (clone 26G6) | WB, ELISA, FC, IF, IHC | Mouse IgG1 |
| MRO-0350-CN | Rabbit Anti-CEBPA Recombinant Antibody (clone CBACN-145) | WB, IP, FC | Rabbit IgG |
| VS3-WK59 | Mouse Anti-CEBPA Recombinant Antibody (clone E8-B7) | WB, ELISA | Mouse IgG1 |
| VS3-CJ991 | Rabbit Anti-CEBPA Recombinant Antibody (VS3-CJ991) | WB, IP, FC | Rabbit IgG |
| VS3-XY490 | Mouse Anti-CEBPA Recombinant Antibody (clone 4E10) | ELISA, WB, FC | Mouse IgG1 |
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